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ID 57334
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著者
Kato, Mutsuko Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Sato, Keita Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Habuta, Munenori Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Fujita, Hirofumi Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Bando, Tetsuya Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Morizane, Yuki Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science
Shiraga, Fumio Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science
Miyaishi, Satoru Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ohuchi, Hideyo Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
抄録
Recent studies show that exposure to ultraviolet (UV) light suppresses ocular elongation, which causes myopia development. However, the specific mechanisms of this process have not been elucidated. A UV-sensor, Opsin 5 (Opn5) mRNA was shown to be present in extraretinal tissues. To test the possibility that UV-signals mediated by Opn5 would have a direct effect on the outer connective tissues of the eye, we first examined the expression patterns of a mammalian type Opn5 (Opn5m) in the late-embryonic chicken eye. Quantitative PCR showed Opn5m mRNA expression in the cornea and sclera. The anti-Opn5m antibody stained a small subset of cells in the corneal stroma and fibrous sclera. We next assessed the effect of UV-A (375 nm) irradiation on the chicken fibroblast cell line DF-1 overexpressing chicken Opn5m. UV-A irradiation for 30 min significantly increased the expression of Early growth response 1 (Egr1), known as an immediate early responsive gene, and of Matrix metalloproteinase 2 (Mmp2) in the presence of retinal chromophore 11-cis-retinal. In contrast, expression of Transforming growth factor beta 2 and Tissue inhibitor of metalloproteinase 2 was not significantly altered. These results indicate that UV-A absorption by Opn5m can upregulate the expression levels of Egr1 and Mmp2 in non-neuronal, fibroblasts. Taken together with the presence of Opn5m in the cornea and sclera, it is suggested that UV-A signaling mediated by Opn5 in the extraretinal ocular tissues could influence directly the outer connective tissues of the chicken late-embryonic eye.
キーワード
Opsin 5
UV-Absorbing pigment
Fibroblasts
Chicken
Egr1
Mmp2
発行日
2019-09-30
出版物タイトル
Biochemistry and Biophysics Reports
19巻
出版者
Elsevier B.V
開始ページ
100665
ISSN
24055808
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
© 2019 The Authors. Published by Elsevier B.V.
論文のバージョン
publisher
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DOI
関連URL
isVersionOf https://doi.org/10.1016/j.bbrep.2019.100665
ライセンス
http://creativecommons.org/licenses/by-nc-nd/4.0/
Citation
Mutsuko Kato, Keita Sato, Munenori Habuta, Hirofumi Fujita, Tetsuya Bando, Yuki Morizane, Fumio Shiraga, Satoru Miyaishi, Hideyo Ohuchi, Localization of the ultraviolet-sensor Opn5m and its effect on myopia-related gene expression in the late-embryonic chick eye, Biochemistry and Biophysics Reports, Volume 19, 2019, 100665, ISSN 2405-5808, https://doi.org/10.1016/j.bbrep.2019.100665.
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