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ID 53967
フルテキストURL
著者
Hamada, Yoshimasa Graduate School of Natural Science and Technology, Okayama University
Bando, Tetsuya Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Nakamura, Taro Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School
Ishimaru, Yoshiyasu Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School
Mito, Taro Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School
Noji, Sumihare Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School
Tomioka, Kenji Graduate School of Natural Science and Technology, Okayama University
Ohuchi, Hideyo Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
抄録
Hemimetabolous insects such as the cricket Gryllus bimaculatus regenerate lost tissue parts using blastemal cells, a population of dedifferentiated proliferating cells. The expression of several factors that control epigenetic modification is upregulated in the blastema compared with differentiated tissue, suggesting that epigenetic changes in gene expression might control the differentiation status of blastema cells during regeneration. To clarify the molecular basis of epigenetic regulation during regeneration, we focused on the function of the Gryllus Enhancer of zeste [Gb'E(z)] and Ubiquitously transcribed tetratricopeptide repeat gene on the X chromosome (Gb'Utx) homologues, which regulate methylation and demethylation of histone H3 lysine 27 (H3K27), respectively. Methylated histone H3K27 in the regenerating leg was diminished by Gb'E(z)RNAi and was increased by Gb'UtxRNAi. Regenerated Gb'E(z)RNAi cricket legs exhibited extra leg segment formation between the tibia and tarsus, and regenerated Gb'UtxRNAi cricket legs showed leg joint formation defects in the tarsus. In the Gb'E(z)RNAi regenerating leg, the Gb'dac expression domain expanded in the tarsus. By contrast, in the Gb'UtxRNAi regenerating leg, Gb'Egfr expression in the middle of the tarsus was diminished. These results suggest that regulation of the histone H3K27 methylation state is involved in the repatterning process during leg regeneration among cricket species via the epigenetic regulation of leg patterning gene expression.
キーワード
Regeneration
Epigenetics
Histone H3K27
Gryllus bimaculatus
Polycomb
発行日
2015-09
出版物タイトル
Development
142巻
17号
出版者
Company of Biologists
開始ページ
2916
終了ページ
2927
ISSN
0950-1991
NCID
AA10667805
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1242/dev.122598
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/53950
言語
English
著作権者
© 2015. The authors, Publication licensee The Company of Biologists Ltd
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Web of Sience KeyUT