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ID 56680
フルテキストURL
著者
Dhira Rani Saha Division of Histology & Electron microscopy, National Institute of Cholera and Enteric Diseases
Guin, Sucharita Natl Inst Cholera & Enter Dis, Div Bacteriol
Krishnan, Rajendran Natl Inst Cholera & Enter Dis, Div Data Management
Nag, Dhrubajyoti Natl Inst Cholera & Enter Dis, Div Bacteriol
Koley, Hemanta Natl Inst Cholera & Enter Dis, Div Bacteriol
Shinoda, Sumio Okayama Univ Infect Dis India, Collaborat Res Ctr
Ramamurthy, Thandavarayan Natl Inst Cholera & Enter Dis, Div Bacteriol
抄録
Background  This study was conducted to determine the role of enteroaggregative Escherichia coli (EAEC) in inflammatory diarrhea among hospitalized patients in Kolkata. The inflammatory pathogenesis of EAEC was established in mice model and histopathological studies. Presence of fecal leucocytes (FLCs) can be suspected for EAEC infection solely or as a mixed with other enteric pathogens.  Methods  Active surveillance was conducted for 2 years on 2 random days per week with every 5th patient admitted to the Infectious Diseases Hospital (IDH). Diarrheal samples were processed by conventional culture, microscopy, ELISA and molecular methods. Two EAEC isolated as sole pathogens were examined in mice after induced intestinal infection. The intestinal tissue samples were processed to analyze the histological changes.  Results  Of the 2519 samples screened, fecal leucocytes, erythrocytes and occult blood were detected in 1629 samples. Most of the patients had acute watery diarrhea (75%) and vomiting (78%). Vibrio cholerae O1 was the main pathogen in patients of 5–10 years age group (33%). Shigellosis was more in children from 2–5 years of age (19%), whereas children <2 years appeared to be susceptible for infection caused by EAEC (16%). When tested for the pathogenicity, the EAEC strains colonized well and caused inflammatory infection in the gut mucosa of BALB/C mice.  Conclusion  This hospital-based surveillance revealed prevalence of large number of inflammatory diarrhea. EAEC was the suspected pathogen and <2 years children appeared to be the most susceptible age group. BALB/C mice may be a suitable animal model to study the EAEC-mediated pathogenesis.
発行日
2013-12-03
出版物タイトル
Gut Pathogens
5巻
1号
出版者
BioMed Central
開始ページ
36
ISSN
17574749
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
publisher
PubMed ID
DOI
Web of Sience KeyUT
関連URL
isVersionOf https://doi.org/10.1186/1757-4749-5-36
プロジェクト
インド感染症共同研究