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ID 32996
フルテキストURL
著者
Moritani, Norifumi H Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine and Dentistry
Kubota, Satoshi Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine and Dentistry
Sugahara, Toshio Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine and Dentistry
Takigawa, Masaharu Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine and Dentistry
抄録

Background: The chondrosarcoma-derived HCS-2/8 has been known to be an excellent model of human articular chondrocytes. By mimicking the arthritic conditions through the treatment of HCS-2/8 cells with cytokines, we estimated the gene expression response of ccn1 and ccn2 during the course of joint inflammation in vitro.
Results: In order to mimic the initiation of inflammation, HCS-2/8 cells were treated with tumor necrosis factor (TNF)-α. To induce pro-inflammatory or reparative responses, TGF-β was employed. Effects of an anti-inflammatory glucocorticoid were also evaluated. After stimulation, expression levels of ccn1 and ccn2 were quantitatively analyzed. Surprisingly, not only ccn2, but also ccn1 expression was repressed upon TNF-α stimulation, whereas both mRNAs were uniformly induced by transforming growth factor (TGF)-β and a glucocorticoid.
Conclusion: These results describing the same response during the course of inflammation suggest similar and co-operative roles of these 2 ccn family members in the course of arthritis.

備考
Digital Object Identifier:10.1186/1478-811X-3-6
Published with permission from the copyright holder. This is the institute's copy, as published in Cell Communication and Signaling, Volume 3, 15 April 2005, Article number 6.
Publisher URL:http://dx.doi.org/10.1186/1478-811X-3-6
Copyright © 2005 Moritani et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
発行日
2005-04-15
出版物タイトル
Cell Communication and Signaling
3巻
1号
出版者
BioMed Central
NCID
AA12050544
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
Moritani et al; licensee BioMed Central Ltd.
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Submission Path
biology_general/1