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ID 53885
フルテキストURL
著者
Kitamura, Shinji Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Sakurai, Hiroyuki Department of Pharmacology and Toxicology, Kyorin University School of Medicine
Makino, Hirofumi Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
抄録
The kidneys are formed during development from two distinct primordial tissues, the metanephric mesenchyme and the ureteric bud. The metanephric mesenchyme develops into the kidney nephron, the minimal functional unit of the kidney. A nephron consists of several segments and regulates water, electrolyte, and acid-base homeostasis in addition to secreting certain hormones. It has been predicted that the kidney will be among the last organs successfully regenerated in vitro due to its complex structure and multiple functions. Here, we show that adult kidney stem/progenitor cells (KS cells), derived from the S3 segment of adult rat kidney nephrons, can reconstitute a three-dimensional kidney-like structure in vitro. Kidney-like structures were formed when a cluster of KS cells was suspended in an extracellular matrix gel and cultured in the presence of several growth factors. Morphological analyses revealed that these kidney-like structures contained every substructure of the kidney, including glomeruli, proximal tubules, the loop of Henle, distal tubules, and collecting ducts, but no vasculature. Our results demonstrate that a cluster of tissue stem/progenitor cells has the ability to reconstitute the minimum unit of its organ of origin by differentiating into specialized cells in the correct location. This process differs from embryonic kidney development, which requires the mutual induction of two different populations of progenitors, metanephric mesenchymal cells and ureteric bud cells.
キーワード
Adult kidney tissue stem cells
Three-dimensional nephron structures in vitro
Organogenesis
備考
"This is the peer reviewed version of the following article: [Kitamura, S., Sakurai, H. and Makino, H. (2015), Single Adult Kidney Stem/Progenitor Cells Reconstitute Three-Dimensional Nephron Structures In Vitro. STEM CELLS, 33: 774–784. doi: 10.1002/stem.1891], which has been published in final form at [http://dx.doi.org/10.1002/stem.1891]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."
発行日
2015-03
出版物タイトル
Stem cells
33巻
3号
出版者
AlphaMed Press
開始ページ
774
終了ページ
784
ISSN
1066-5099
NCID
AA10876157
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1002/stem.1891
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/53883
言語
English
OAI-PMH Set
岡山大学
著作権者
© 2014 AlphaMed Press
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査読
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