ID | 56699 |
フルテキストURL | |
著者 |
Ghosh, Priyanka
Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Kumar, Dhirendra
Maharishi Valmiki Infectious Diseases Hospital
Chowdhury, Goutam
Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Singh, Puneeta
Maharishi Valmiki Infectious Diseases Hospital
Samanta, Prosenjit
Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Dutta, Shanta
Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Ramamurthy, T.
Division of Bacteriology, National Institute of Cholera and Enteric Diseases
Sharma, N. C.
Maharishi Valmiki Infectious Diseases Hospital
Sinha, Preety
Department of Zoology, A.N. College
Prasad, Yogendra
Department of Animal Science, MJP Rohilkhand University
Shinoda, Sumio
Collaborative Research Center of Okayama University for Infectious Diseases at NICED
Mukhopadhyay, Asish K.
Division of Bacteriology, National Institute of Cholera and Enteric Diseases
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抄録 | Vibrio cholerae O1 is the etiological agent of the severe diarrheal disease cholera. The bacterium has recently been causing outbreaks in Haiti with catastrophic effects. Numerous mutations have been reported in V. cholerae O1 strains associated with the Haitian outbreak. These mutations encompass among other the genes encoding virulence factors such as the pilin subunit of the toxin-co-regulated pilus (tcpA), cholera toxin B subunit (ctxB), repeat in toxins (rtxA), and other genes such as the quinolone resistance-determining region (QRDR) of gyrase A (gyrA), rstB of RS element along with the alteration in the number of repeat sequences at the promoter region of ctxAB. Given the numerous genetic changes in those Haitian isolates, we decided to investigate the possible origins of those variations in the Indian subcontinent. Thus, we determined the genetic traits among V. cholerae O1 strains in Delhi, India. A total of 175 strains isolated from cholera patients during 2004 to 2012 were analysed in the present study. Our results showed that all the tested strains carried Haitian type tcpA (tcpACIRS) and variant gyrA indicating their first appearance before 2004 in Delhi. The Haitian variant rtxA and ctxB7 were first detected in Delhi during 2004 and 2006, respectively. Interestingly, not a single strain with the combination of El Tor rtxA and ctxB7 was detected in this study. The Delhi strains carried four heptad repeats (TTTTGAT) in the CT promoter region whereas Haitian strains carried 5 such repeats. Delhi strains did not have any deletion mutations in the rstB like Haitian strains. Overall, our study demonstrates the sequential accumulation of Haitian-like genetic traits among V. cholerae O1 strains in Delhi at different time points prior to the Haitian cholera outbreak.
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キーワード | Cholera
Vibrio cholerae
ctxAB promoter
ctxB
gyrA
rstB
rtxA
tcpA
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発行日 | 2017-10
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出版物タイトル |
Infection, Genetics and Evolution
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巻 | 54巻
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出版者 | Elsevier Science
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開始ページ | 47
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終了ページ | 53
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ISSN | 15671348
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NCID | AA11697619
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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論文のバージョン | author
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1016/j.meegid.2017.06.015
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プロジェクト |
インド感染症共同研究
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