ID | 48278 |
フルテキストURL | |
著者 |
Miyoshi, Shin-ichi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kaken ID
publons
researchmap
Abe, Yuki
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Senoh, Mitsutoshi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Mizuno, Tamaki
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
researchmap
Maehara, Yoko
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Nakao, Hiroshi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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抄録 | Vibrio vulnificus is an etiological agent causing serious systemic infections in the immunocompromised humans or cultured eels. This species commonly produces a hemolytic toxin consisting of the cytolysin domain and the lectin-like domain. For hemolysis, the lectin-like domain specifically binds to cholesterol in the erythrocyte membrane, and to form a hollow oligomer, the toxin is subsequently assembled on the membrane. The cytolysin domain is essential for the process to form the oligomer. Three-dimensional structure model revealed that two domains connected linearly and the C-terminus was located near to the joint of the domains. Insertion of amino acid residues between two domains was found to cause inactivation of the toxin. In the C-terminus, deletion, substitution or addition of an amino acid residue also elicited reduction of the activity. However, the cholesterol-binding ability was not affected by the mutations. These results suggest that mutation of the C- or N-terminus of the lectin-like domain may result in blockage of the toxin assembly.
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キーワード | Vibrio vulnificus
Hemolysin
Cell-free translation
Site-directed mutagenesis
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発行日 | 2011-05
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出版物タイトル |
Toxicon
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巻 | 57巻
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号 | 6号
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出版者 | Elsevier
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開始ページ | 904
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終了ページ | 908
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ISSN | 0041-0101
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NCID | AA00864479
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資料タイプ |
学術雑誌論文
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プロジェクト |
インド感染症共同研究
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オフィシャル URL | http://www.sciencedirect.com/science/article/pii/S0041010111000997
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言語 |
英語
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著作権者 | ©2011 Elsevier Ltd. All rights reserved.
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論文のバージョン | author
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査読 |
有り
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DOI | |
PubMed ID | |
Web of Science KeyUT |