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ID 48278
フルテキストURL
著者
Miyoshi, Shin-ichi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Abe, Yuki Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Senoh, Mitsutoshi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Mizuno, Tamaki Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Maehara, Yoko Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Nakao, Hiroshi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
抄録
Vibrio vulnificus is an etiological agent causing serious systemic infections in the immunocompromised humans or cultured eels. This species commonly produces a hemolytic toxin consisting of the cytolysin domain and the lectin-like domain. For hemolysis, the lectin-like domain specifically binds to cholesterol in the erythrocyte membrane, and to form a hollow oligomer, the toxin is subsequently assembled on the membrane. The cytolysin domain is essential for the process to form the oligomer. Three-dimensional structure model revealed that two domains connected linearly and the C-terminus was located near to the joint of the domains. Insertion of amino acid residues between two domains was found to cause inactivation of the toxin. In the C-terminus, deletion, substitution or addition of an amino acid residue also elicited reduction of the activity. However, the cholesterol-binding ability was not affected by the mutations. These results suggest that mutation of the C- or N-terminus of the lectin-like domain may result in blockage of the toxin assembly.
キーワード
Vibrio vulnificus
Hemolysin
Cell-free translation
Site-directed mutagenesis
発行日
2011-05
出版物タイトル
Toxicon
57巻
6号
出版者
Elsevier
開始ページ
904
終了ページ
908
ISSN
0041-0101
NCID
AA00864479
資料タイプ
学術雑誌論文
プロジェクト
インド感染症共同研究
オフィシャル URL
http://www.sciencedirect.com/science/article/pii/S0041010111000997
言語
English
OAI-PMH Set
岡山大学
著作権者
©2011 Elsevier Ltd. All rights reserved.
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査読
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DOI
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