ID | 52119 |
フルテキストURL | |
著者 |
Muraoka, Takayuki
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Soh, Junichi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
ORCID
Kaken ID
researchmap
Toyooka, Shinichi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac SurgPathol
Aoe, Keisuke
Yamaguchi Ube Med Ctr, Natl Hosp Org, Dept Med Oncol & Clin Res
Fujimoto, Nobukazu
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Canc & Thorac Surg
Hashida, Shinsuke
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Maki, Yuho
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Tanaka, Norimitsu
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Shien, Kazuhiko
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
ORCID
Kaken ID
publons
researchmap
Furukawa, Masashi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Yamamoto, Hiromasa
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
ORCID
Kaken ID
publons
researchmap
Asano, Hiroaki
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Kaken ID
Tsukuda, Kazunori
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
ORCID
Kaken ID
publons
researchmap
Kishimoto, Takumi
Okayama Rosai Hosp, Dept Resp Med
Otsuki, Takemi
Kawasaki Med Univ, Dept Hyg
Miyoshi, Shinichiro
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Kaken ID
publons
researchmap
|
抄録 | Objectives: Malignant pleural mesothelioma (MPM) is an aggressive tumor with a poor prognosis. microRNA-34b/c (miR-34b/c), which plays an important role in the pathogenesis of MPM, is frequently downregulated by DNA methylation in approximately 90% of MPM cases. In this study, we estimated the degree of miR-34b/c methylation in serum-circulating DNA using a digital methylation specific PCR assay (MSP).
Materials and methods: A real-time MSP assay was performed using the SYBR Green method. The melting temperature (Tm) of each PCR product was examined using a melting curve analysis. For a digital MSP assay, 40 wells were analyzed per sample. A total of 110 serum samples from 48 MPM cases, 21 benign asbestos pleurisy (BAP) cases, and 41 healthy volunteers (HVs) were examined.
Results: Positive range of Tm value for miR-34b/c methylation was defined as 77.71-78.79 degrees C which was the mean 3 standard deviations of 40 wells of a positive control. The number of miR-34b/c methylated wells was counted per sample according to this criterion. The number of miR-34b/c methylated wells in MPM cases was significantly higher than that in BAP cases (P = 0.03) or HVs (P < 0.001). Advanced MPM cases tended to have higher number of miR-34b/c methylated wells than early MPM cases. Receiver-operating characteristic (ROC) curve analysis revealed that three number of miR-34b/c methylated wells per sample was the best cut-off of positivity of MPM with a 67% of sensitivity and a 77% specificity for prediction. The area under the ROC curve was 0.77.
Conclusions: Our digital MSP assay can quantify miR-34b/c methylation in serum-circulating DNA. The degree of miR-34b/c methylation in serum-circulating DNA is associated with MPM, suggesting that this approach might be useful for the establishment of a new detection system for MPM.
|
キーワード | Digital PCR
Malignant pleural mesothelioma
microRNA
miR-34b/c
Methylation
Circulating DNA
|
発行日 | 2013-12
|
出版物タイトル |
Lung Cancer
|
巻 | 82巻
|
号 | 3号
|
出版者 | Elsevier Ireland Ltd.
|
開始ページ | 485
|
終了ページ | 490
|
ISSN | 0169-5002
|
NCID | AA10785743
|
資料タイプ |
学術雑誌論文
|
オフィシャル URL | http://dx.doi.org/10.1016/j.lungcan.2013.09.017
|
関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/52509
|
言語 |
英語
|
著作権者 | (C) 2013 Elsevier Ireland Ltd. All rights reserved.
|
論文のバージョン | author
|
査読 |
有り
|
DOI | |
Web of Science KeyUT |