ID | 65543 |
フルテキストURL | |
著者 |
Jaunet-Lahary, Titouan
Research Center for Computational Science, Institute for Molecular Science, National Institutes of Natural Sciences
Shimamura, Tatsuro
Graduate School of Medicine, Kyoto University
Hayashi, Masahiro
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Nomura, Norimichi
Graduate School of Medicine, Kyoto University
Hirasawa, Kouta
Graduate School of Medicine, Kyoto University
Shimizu, Tetsuya
RIKEN SPring-8 Center
Yamashita, Masao
RIKEN SPring-8 Center
Tsutsumi, Naotaka
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Suehiro, Yuta
School of Pharmaceutical Sciences, Okayama University
Kojima, Keiichi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Sudo, Yuki
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Tamura, Takashi
Graduate School of Environmental and Life Sciences, Okayama University
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Iwanari, Hiroko
Research Center for Advanced Science and Technology, The University of Tokyo
Hamakubo, Takao
Research Center for Advanced Science and Technology, The University of Tokyo
Iwata, So
Graduate School of Medicine, Kyoto University
Okazaki, Kei-Ichi
Research Center for Computational Science, Institute for Molecular Science, National Institutes of Natural Sciences
Hirai, Teruhisa
RIKEN SPring-8 Center
Yamashita, Atsuko
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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抄録 | An oxalate-degrading bacterium in the gut microbiota absorbs food-derived oxalate to use this as a carbon and energy source, thereby reducing the risk of kidney stone formation in host animals. The bacterial oxalate transporter OxlT selectively uptakes oxalate from the gut to bacterial cells with a strict discrimination from other nutrient carboxylates. Here, we present crystal structures of oxalate-bound and ligand-free OxlT in two distinct conformations, occluded and outward-facing states. The ligand-binding pocket contains basic residues that form salt bridges with oxalate while preventing the conformational switch to the occluded state without an acidic substrate. The occluded pocket can accommodate oxalate but not larger dicarboxylates, such as metabolic intermediates. The permeation pathways from the pocket are completely blocked by extensive interdomain interactions, which can be opened solely by a flip of a single side chain neighbouring the substrate. This study shows the structural basis underlying metabolic interactions enabling favourable symbiosis.
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備考 | The version of record of this article, first published in Nature Communications, is available online at Publisher’s website: http://dx.doi.org/10.1038/s41467-023-36883-5
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発行日 | 2023-04-03
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出版物タイトル |
Nature Communications
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巻 | 14巻
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号 | 1号
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出版者 | Nature Portfolio
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開始ページ | 1730
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ISSN | 2041-1723
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © The Author(s) 2023
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1038/s41467-023-36883-5
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ライセンス | http://creativecommons.org/licenses/by/4.0/
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Citation | Jaunet-Lahary, T., Shimamura, T., Hayashi, M. et al. Structure and mechanism of oxalate transporter OxlT in an oxalate-degrading bacterium in the gut microbiota. Nat Commun 14, 1730 (2023). https://doi.org/10.1038/s41467-023-36883-5
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助成機関名 |
Japan Society for the Promotion of Science
Japan Agency for Medical Research and Development
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助成番号 | JP20H03195
JP18H02415
JP26440086
JP20am0101079
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