ID | 58555 |
フルテキストURL | |
著者 |
Yoshida, Ryusuke
Section of Oral Neuroscience, Graduate School of Dental Science, Kyushu University
ORCID
Kaken ID
researchmap
Takai, Shingo
Section of Oral Neuroscience, Graduate School of Dental Science, Kyushu University
Sanematsu, Keisuke
Section of Oral Neuroscience, Graduate School of Dental Science, Kyushu University
Margolskee, Robert F.
Monell Chemical Senses Center
Shigemura, Noriatsu
Section of Oral Neuroscience, Graduate School of Dental Science, Kyushu University
Ninomiya, Yuzo
Section of Oral Neuroscience, Graduate School of Dental Science, Kyushu University
|
抄録 | Bitter taste serves as an important signal for potentially poisonous compounds in foods to avoid their ingestion. Thousands of compounds are estimated to taste bitter and presumed to activate taste receptor cells expressing bitter taste receptors (Tas2rs) and coupled transduction components including gustducin, phospholipase Cβ2 (PLCβ2) and transient receptor potential channel M5 (TRPM5). Indeed, some gustducin-positive taste cells have been shown to respond to bitter compounds. However, there has been no systematic characterization of their response properties to multiple bitter compounds and the role of transduction molecules in these cells. In this study, we investigated bitter taste responses of gustducin-positive taste cells in situ in mouse fungiform (anterior tongue) and circumvallate (posterior tongue) papillae using transgenic mice expressing green fluorescent protein in gustducin-positive cells. The overall response profile of gustducin-positive taste cells to multiple bitter compounds (quinine, denatonium, cyclohexamide, caffeine, sucrose octaacetate, tetraethylammonium, phenylthiourea, L-phenylalanine, MgSO4, and high concentration of saccharin) was not significantly different between fungiform and circumvallate papillae. These bitter-sensitive taste cells were classified into several groups according to their responsiveness to multiple bitter compounds. Bitter responses of gustducin-positive taste cells were significantly suppressed by inhibitors of TRPM5 or PLCβ2. In contrast, several bitter inhibitors did not show any effect on bitter responses of taste cells. These results indicate that bitter-sensitive taste cells display heterogeneous responses and that TRPM5 and PLCβ2 are indispensable for eliciting bitter taste responses of gustducin-positive taste cells.
|
キーワード | bitter antagonists
bitter receptor
breadth of responsiveness
taste coding
transgenic mouse
|
発行日 | 2018-01-15
|
出版物タイトル |
Neuroscience
|
巻 | 369巻
|
出版者 | Elsevier
|
開始ページ | 29
|
終了ページ | 39
|
ISSN | 0306-4522
|
NCID | AA0075489X
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
OAI-PMH Set |
岡山大学
|
論文のバージョン | author
|
PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1016/j.neuroscience.2017.10.047
|
ライセンス | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
|
Citation | Yoshida R, Takai S, Sanematsu K, Margolskee RF, Shigemura N, Ninomiya Y. Bitter Taste Responses of Gustducin-positive Taste Cells in Mouse Fungiform and Circumvallate Papillae. Neuroscience. 2018;369:29‐39. doi:10.1016/j.neuroscience.2017.10.047
|
助成機関名 |
日本学術振興会
|
助成番号 | 23689076
26462815
26670810
15H02571
|