ID | 31099 |
JaLCDOI | |
フルテキストURL | |
著者 |
Umeda, Mamoru
Nissui Pharmaceutical company limited
Yasuda, Tatsuji
Okayama University
Kaken ID
|
抄録 | We have already developed the liposome immune lysis assay (LILA) for the determination of C-reactive protein (CRP) by employing an inhibition method and a sandwich method. We herein report a new LILA system involving the use of monoclonal antibodies-bearing liposomes. We established five monoclonal antibodies to CRP antigen, AC-1, -2, -3, -4, -5 which had the capacity to activate complement and form antigen-antibody complex. Each of these antibodies was covalently coupled to carboxyfluorescein-entrapped multilamellar liposomes. When the liposomes were incubated with CRP antigen in the presence of guinea pig complement, CRP antigen-dependent liposome lysis was observed but the sensitivity was not great enough for practical use. On the other hand, when liposomes coupling two monoclonal antibodies (AC-1, AC-2) which recognized distinct CRP antigenic determinants were employed in the assay, the sensitivity increased compared with that using only one monoclonal antibody, and the detectable concentration range was 5-300 ng/ml. These results indicated that the combination of two or more monoclonal antibodies which recognize distinct CRP antigenic determinants is effective for increasing the sensitivity of the assay. |
キーワード | liposome immune lysis assay
C-reactive protein
carboxyfluoescein
mouse monoclonal antibodies
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 1994-12
|
巻 | 48巻
|
号 | 6号
|
出版者 | Okayama University Medical School
|
開始ページ | 299
|
終了ページ | 304
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |