ID | 54548 |
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著者 |
Ohtsuki, Takashi
Department of Biomedical Engineering, Okayama University
ORCID
Kaken ID
publons
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Kanzaki, Shigeto
Department of Biomedical Engineering, Okayama University
Nishimura, Sae
Department of Biomedical Engineering, Okayama University
Kunihiro, Yoshio
Department of Biomedical Engineering, Okayama University
Sisido, Masahiko
Department of Biomedical Engineering, Okayama University
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抄録 | The possibility of spatiotemporally photocontrolling translation holds considerable promise for studies on the biological roles of local translation in cells and tissues. Here we report caged aminoacyl-tRNAs (aa-tRNAs) synthesized using a (7-diethylaminocoumarin-4-yl)methoxycarbonyl (DEACM)-cage compound. DEACM-caged aa-tRNA does not spontaneously deacylate for at least 4 h in neutral aqueous solution, and does not bind to the elongation factor Tu. On irradiation at ∼405 nm at 125 mW cm(-2), DEACM-aa-tRNA is converted into active aa-tRNA with a half-life of 19 s. Notably, this rapid uncaging induced by visible light does not impair the translation system. Translation is photoinduced when DEACM-aa-tRNA carrying a CCCG or a CUA anticodon is uncaged in the presence of mRNAs harbouring a CGGG four-base codon or a UAG amber codon, respectively. Protein synthesis is phototriggered in several model systems, including an in vitro translation system, an agarose gel, in liposomes and in mammalian cells.
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備考 | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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発行日 | 2016-08
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出版物タイトル |
Nature Communications
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巻 | 7巻
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出版者(別表記) | Macmillan Publishers Limited, part of Springer Nature
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開始ページ | 12501
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終了ページ | 12501
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ISSN | 2041-1723
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NCID | AA12645905
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://www.nature.com/ncomms/
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言語 |
英語
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著作権者 | © The Author(s) 2016
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論文のバージョン | publisher
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査読 |
有り
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DOI | |
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