ID | 50977 |
フルテキストURL | |
著者 |
Koreishi, Mayuko
Okayama Univ, Grad Sch Nat Sci & Technol
Gniadek, Thomas J.
Johns Hopkins Univ, Sch Med, Dept Pathol
Yu, Sidney
Chinese Univ Hong Kong, Sch Biomed Sci, Shatin
Masuda, Junko
NIAID, Mucosal Immun Sect, Lab Host Def
Honjo, Yasuko
Okayama Univ, RCIS
|
抄録 | Golgins are coiled-coil proteins that play a key role in the regulation of Golgi architecture and function. Giantin, the largest golgin in mammals, forms a complex with p115, rab1, GM130, and soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), thereby facilitating vesicle tethering and fusion processes around the Golgi apparatus. Treatment with the microtubule destabilizing drug nocodazole transforms the Golgi ribbon into individual Golgi stacks. Here we show that siRNA-mediated depletion of giantin resulted in more dispersed Golgi stacks after nocodazole treatment than by control treatment, without changing the average cisternal length. Furthermore, depletion of giantin caused an increase in cargo transport that was associated with altered cell surface protein glycosylation. Drosophila S2 cells are known to have dispersed Golgi stacks and no giantin homolog. The exogenous expression of mammalian giantin cDNA in S2 cells resulted in clustered Golgi stacks, similar to the Golgi ribbon in mammalian cells. These results suggest that the spatial organization of the Golgi ribbon is mediated by giantin, which also plays a role in cargo transport and sugar modifications.
|
発行日 | 2013-05-21
|
出版物タイトル |
PLoS ONE
|
巻 | 8巻
|
号 | 3号
|
出版者 | Public Library Science
|
ISSN | 1932-6203
|
資料タイプ |
学術雑誌論文
|
オフィシャル URL | http://dx.doi.org/10.1371/journal.pone.0059821
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
DOI | |
Web of Science KeyUT |