フルテキストURL fulltext.pdf
著者 Ikeda, Risa| Kurokawa, Miki| Murai, Momoka| Saito, Noboru| Ando, Motonori|
キーワード heliozoa immunocytochemistry microtubule glutaraldehyde confocal microscopy
発行日 2020
出版物タイトル Acta Protozoologica
59巻
1号
開始ページ 1
終了ページ 12
ISSN 0065-1583
NCID AA00510002
資料タイプ 学術雑誌論文
言語 英語
OAI-PMH Set 岡山大学
論文のバージョン publisher
DOI 10.4467/16890027AP.20.001.12157
Web of Science KeyUT 000605760600001
タイトル(別表記) Cytoskeletal elements in an acoelomorph worm, Praesagittifera naikaiensis
フルテキストURL poalas_034_021_180706.pdf
著者 池田 理佐| 藤原 稚穂| 濱田 麻友子| 坂本 竜哉| 齋藤 昇| 安藤 元紀|
抄録 Acoel flatworms can move in a variety of ways such as muscular and ciliary movements via cytoskeletal elements and their neural regulations. However, those locomotive mechanisms have not yet been fully elucidated. In this study, we examined the distribution of cytoskeletal elements including filamentous actin (F-actin) and tubulin, and the neuroanatomical organization in an acoelomorph worm, Praesagittifera naikaiensis (P. naikaiensis). Video microscopy revealed the elongation/contraction and the bending/rotation processes, and the ciliary gliding movement of P. naikaiensis. Histochemical and morphological analysis demonstrated that F-actin networks of inner longitudinal and outer circular muscle fibers were positioned along the entire surface of the body, and that the average distance between the circular muscle fibers in the contracted organism was decreased in the anterior region compared with that in the elongated organism. Electron microscopy showed dense bodies on the muscle cells of P. naikaiensis, which indicates that those muscle cells have the appearance of vertebrate smooth muscle cells. Immunohistochemical analysis revealed that -tubulin-positive signals on the ciliary microtubules had close contact with the F-actin network, and that neurite bundles labelled with anti dSap47 antibody as a neuronal marker run along the anterior-posterior body axis. These results indicate that the well-organized cytoskeletal elements and their neural control systems are preserved in P. naikaiensis, and that their mechanisms involved in those regulation systems are similar to those vertebrate systems. Further studies are needed to clarify the physiological mechanisms underlying the muscular and ciliary movements in P. naikaiensis.
出版物タイトル 岡山実験動物研究会報
発行日 2018-04
34巻
開始ページ 21
終了ページ 27
言語 日本語
論文のバージョン publisher
NAID 120006469118
タイトル(別表記) Structure and function of tegmentum vasculosum in avian cochlea
フルテキストURL poalas_033_026_030.pdf
著者 池田 理佐| 乙野 剛史| 池田 直矢| 齋藤 昇| 安藤 元紀|
抄録 In spite of the importance of endocochlear DC potential (EP) and the K+-rich endolymph for the avian cochlea, the structure and function of the tegmentum vasculosum (TV) has not yet been fully elucidated, compared with those of the stria vascularis in the mammalian cochlea. In this study, we examined structural analysis of the epithelial cells, gene expressions of Na+-K+-ATPase (Atp1A1) and Kir4.1 (Kcnj10), and protein localizations of Na+-K+-ATPase and Kir4.1, in the TV. Tight junctional structures were observed between epithelial dark cells and light cells in the TV. Both Atp1a1 and Kcnj10 genes were detected in the TV. In addition, immunopositive signals for both Na+-K+-ATPase and Kir4.1 were recognized in the TV. These results indicate that Na+-K+-ATPase and Kir4.1 play roles in maintaining the EP and high K+ concentration of the endolymph. Further studies are needed to clarify the physiological functions of the TV. This is the first report which demonstrates that gene and protein expression data contribute to the avian inner ear homeostasis.
出版物タイトル 岡山実験動物研究会報
発行日 2017-04
33巻
開始ページ 26
終了ページ 30
言語 日本語
論文のバージョン publisher
著者 齋藤 昇|
発行日 2015-02-01
出版物タイトル 岡山大学農学部学術報告
104巻
資料タイプ 紀要論文
著者 齋藤 昇|
発行日 2014-04
出版物タイトル 岡山実験動物研究会報
30巻
資料タイプ その他