ID | 60448 |
フルテキストURL | |
著者 |
Aulia, Annisa
Institute of Plant Science and Resources, Okayama University
Tabara, Midori
Tokyo University of Agriculture and Technology, Department of Applied Biological Sciences
Telengech, Paul
Institute of Plant Science and Resources, Okayama University
Fukuhara, Toshiyuki
Tokyo University of Agriculture and Technology, Department of Applied Biological Sciences
Suzuki, Nobuhiro
Institute of Plant Science and Resources, Okayama University
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抄録 | The ascomycete Cryphonectria parasitica has served as a model filamentous fungus for studying virus host interactions because of its susceptibility to diverse viruses, its genetic manipulability and the availability of many biological and molecular tools. Cryphonectria prasitica is known to activate antiviral RNA silencing upon infection by some viruses via transcriptional up-regulation of key RNA silencing genes. Here, utilizing a newly developed GFP-based reporter system to monitor dicer-like 2 (dcl2) transcript levels, we show different levels of antiviral RNA silencing activation by different viruses. Some viruses such as mycoreovirus 1, a suppressor-lacking mutant of Cryphonectria hypovirus 1 (CHV1-Δp69) and Rosellinia necatrix partitivirus 11 (RnPV11) highly induced RNA silencing, while others such as CHV3, Rosellinia necatrix victorivirus 1 and RnPV19 did not. There was considerable variation in dcl2 induction by different members within the family Hypoviridae with positive-sense single-stranded RNA genomes or Partitiviridae with double-stranded RNA genomes. Northern blotting and an in vitro Dicer assay developed recently by us using mycelial homogenates validated the reporter assay results for several representative virus strains. Taken together, this study represents a development in the monitoring of Dicer activity in virus-infected C. parasitica.
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キーワード | Dicer
RNA silencing
Fungal virus
RNA virus
Antiviral defense
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発行日 | 2020-07-20
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出版物タイトル |
Current Research in Virological Science
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巻 | 1巻
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出版者 | Elsevier
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開始ページ | 100001
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ISSN | 2666-478X
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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論文のバージョン | publisher
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DOI | |
関連URL | isVersionOf https://doi.org/10.1016/j.crviro.2020.100001
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ライセンス | http://creativecommons.org/licenses/by-nc-nd/4.0/
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オープンアクセス(出版社) |
OA
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オープンアーカイブ(出版社) |
非OpenArchive
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