ID | 62438 |
フルテキストURL | |
著者 |
Otubo, Akito
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Maejima, Sho
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Oti, Takumi
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Satoh, Keita
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
Ueda, Yasumasa
Department of Physiology, Kyoto Prefectural University of Medicine
Morris, John F.
Department of Physiology, Anatomy & Genetics, University of Oxford, South Parks Road
Sakamoto, Tatsuya
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
ORCID
Kaken ID
publons
researchmap
Sakamoto, Hirotaka
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University
ORCID
Kaken ID
publons
researchmap
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抄録 | Translational research often requires the testing of experimental therapies in primates, but research in non-human primates is now stringently controlled by law around the world. Tissues fixed in formaldehyde without glutaraldehyde have been thought to be inappropriate for use in electron microscopic analysis, particularly those of the brain. Here we report the immunoelectron microscopic characterization of arginine vasopressin (AVP)-producing neurons in macaque hypothalamo-pituitary axis tissues fixed by perfusion with 4% formaldehyde and stored at -25 degrees C for several years (4-6 years). The size difference of dense-cored vesicles between magnocellular and parvocellular AVP neurons was detectable in their cell bodies and perivascular nerve endings located, respectively, in the posterior pituitary and median eminence. Furthermore, glutamate and the vesicular glutamate transporter 2 could be colocalized with AVP in perivascular nerve endings of both the posterior pituitary and the external layer of the median eminence, suggesting that both magnocellular and parvocellular AVP neurons are glutamatergic in primates. Both ultrastructure and immunoreactivity can therefore be sufficiently preserved in macaque brain tissues stored long-term, initially for light microscopy. Taken together, these results suggest that this methodology could be applied to the human post-mortem brain and be very useful in translational research.
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キーワード | vasopressin
corticotrophin-releasing factor
glutamate
paraventricular nucleus of the hypothalamus
Japanese macaque monkey
post-embedding immunoelectron microscopy
dense-cored neurosecretory vesicle
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発行日 | 2021-08-25
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出版物タイトル |
International Journal of Molecular Sciences
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巻 | 22巻
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号 | 17号
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出版者 | MDPI
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開始ページ | 9180
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ISSN | 1422-0067
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2021 by the authors.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.3390/ijms22179180
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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Citation | Otubo, A.; Maejima, S.; Oti, T.; Satoh, K.; Ueda, Y.; Morris, J.F.; Sakamoto, T.; Sakamoto, H. Immunoelectron Microscopic Characterization of Vasopressin-Producing Neurons in the Hypothalamo-Pituitary Axis of Non-Human Primates by Use of Formaldehyde-Fixed Tissues Stored at −25 ◦C for Several Years. Int. J. Mol. Sci. 2021, 22, 9180. https:// doi.org/10.3390/ijms22179180
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助成機関名 |
Japan Society for the Promotion of Science
Ministry of Education, Culture, Sports, Science and Technology
Suzuken Memorial Foundation, Japan
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助成番号 | 15K15202
15H05724
15KK025708
16H06280
21H02520
20K15837
21H00428
19-085
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オープンアクセス(出版社) |
OA
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