ID | 55280 |
フルテキストURL |
Supplemental_tables.xlsx
521 KB
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著者 |
Hisano, Hiroshi
Institute of Plant Science and Resources, Okayama University
Meints, Brigid
Department Crop and Soil Sciences, Washington State University
Moscou, Matthew J.
The Sainsbury Laboratory
Cistue, Luis
Department Genetica y Produccion Vegetal
Echávarri, Begoña
Sato, Kazuhiro
Institute of Plant Science and Resources, Okayama University
ORCID
Kaken ID
publons
researchmap
Hayes, Patrick M.
Department Crop and Soil Science, Oregon State University
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抄録 | Key message:
The genetic substitution of transformation amenability alleles from ‘Golden Promise’ can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars.
Abstract:
Barley (Hordeum vulgare) cv. ‘Golden Promise’ is one of the most useful and well-studied cultivars for genetic manipulation. In a previous report, we identified several transformation amenability (TFA) loci responsible for Agrobacterium-mediated transformation using the F2 generation of immature embryos, derived from ‘Haruna Nijo’ × ‘Golden Promise,’ as explants. In this report, we describe higher density mapping of these TFA regions with additional SNP markers using the same transgenic plants. To demonstrate the robustness of transformability alleles at the TFA loci, we genotyped 202 doubled haploid progeny from the cross ‘Golden Promise’ × ‘Full Pint.’ Based on SNP genotype, we selected lines having ‘Golden Promise’ alleles at TFA loci and used them for transformation. Of the successfully transformed lines, DH120366 came the closest to achieving a level of transformation efficiency comparable to ‘Golden Promise.’ The results validate that the genetic substitution of TFA alleles from ‘Golden Promise’ can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars.
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キーワード | Agrobacterium tumefaciens
Doubled haploid
Hordeum vulgare (barley)
Single nucleotide polymorphism
Transformation
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発行日 | 2017-04
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出版物タイトル |
Plant Cell Reports
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巻 | 36巻
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号 | 4号
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出版者 | Springer
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開始ページ | 611
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終了ページ | 620
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ISSN | 0721-7714
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NCID | AA10635864
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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論文のバージョン | author
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1007/s00299-017-2107-2
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