| ID | 48703 |
| フルテキストURL | |
| 著者 |
Shen, Jian-Ren
Graduate School of Natural Science and Technology, Okayama University
ORCID
Kaken ID
publons
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Kawakami, Keisuke
Graduate School of Natural Science and Technology, Okayama University
Koike, Hiroyuki
Department of Biosciences, Faculty of Science and Engineering, Chuo University
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| 抄録 | This chapter describes the purification and crystallization of oxygen-evolving photosystem II core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus. Procedures used for purification of photosystem II from the cyanobacterium involves cultivation of cells, isolation of thylakoid membranes, purification of crude and pure photosystem II core complexes by detergent solubilization, followed by differential centrifugation and column chromatography. The purified core dimer particles were successfully used for crystallization, and the methods and conditions used for crystallization are presented. These purification and crystallization procedures can be applied for another thermophilic cyanobacterium T. elongatus.
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| キーワード | Photosystem II
Oxygen evolution
Crystallization
Membrane proteins
Ion-exchange chromatography
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| 発行日 | 2011
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| 出版物タイトル |
Methods in Molecular Biology
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| 巻 | 684巻
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| 出版者 | Humana Press
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| 開始ページ | 41
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| 終了ページ | 51
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| ISSN | 1064-3745
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| NCID | AA11067778
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| 資料タイプ |
学術雑誌論文
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| プロジェクト |
エネルギー環境新素材拠点
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| オフィシャル URL | http://www.springerlink.com/content/t782187342578257/
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| 言語 |
英語
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| 著作権者 | © Springer Science+Business Media, LLC
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| 論文のバージョン | author
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| 査読 |
有り
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| DOI | |
| PubMed ID |
