ID | 46956 |
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Sort Key | 11
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フルテキストURL | |
著者 |
Yamagiwa, Masashi
Department of Bioscience and Biotechnology Okayama University
Hirao, Taichi
Department of Bioscience and Biotechnology Okayama University
Kiyomi, Masaaki
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
Akao, Tetsuyuki
Biotechnology and Food Research Institute Fukuoka Industrial Technology Center
Mizuki, Eiichi
Biotechnology and Food Research Institute Fukuoka Industrial Technology Center
Ohba, Michio
Graduate School of Agriculture Kyushu University
Sakai, Hiroshi
Department of Bioscience and Biotechnology Okayama University
Kaken ID
publons
researchmap
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抄録 | Bacillus thuringiensis subsp. coreanensis A2316 is a newly isolated strain from Yonakunijima Island in Japan. It produces the proteinaceous inclusion body (crystal) which has no insecticidal and hemolytic activities. When the crystal proteins were digested by proteinase K, they exhibited the strong cytotoxicity against human leukemic T cell, MOLT-4. The proteinase K-digested A2316 crystal proteins have little damage upon the cell membrane of MOLT-4, suggesting that the cell death of MOLT-4 was induced through a mechanism other than the colloid-osmotic swelling and cell lysis as caused by hitherto known B. thuringiensis crystal proteins. The 29-kDa polypeptide proved to be an active component of the proteinase K-digested A2316 crystal proteins. EC(50) of the purified 29-kDa polypeptide was 0.0579 μg/ml. The N-terminal amino acid sequence of the 29-kDa polypeptide was identical with that of p29 produced by B. thuringiensis A1519 strain and shared no significant homology with all the known proteins, suggesting that this polypeptide belong to a new family of B. thuringiensis crystal proteins.
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出版物タイトル |
Memoirs of the Faculty of Engineering, Okayama University
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発行日 | 2004-03
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巻 | 38巻
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号 | 1-2号
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出版者 | Faculty of Engineering, Okayama University
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開始ページ | 97
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終了ページ | 100
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ISSN | 0475-0071
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NCID | AA10699856
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資料タイプ |
紀要論文
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OAI-PMH Set |
岡山大学
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言語 |
英語
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論文のバージョン | publisher
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NAID | |
Eprints Journal Name | mfe
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