このエントリーをはてなブックマークに追加
ID 65157
フルテキストURL
fulltext.pdf 2.85 MB
著者
Sako, Hidefumi Department of Periodontics and Endodontics, Division of Dentistry, Okayama University Hospital
Omori, Kazuhiro Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
Nakayama, Masaaki Department of Oral Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Mandai, Hiroki Department of Pharmacy, Faculty of Pharmacy, Gifu University of Medical Science ORCID Kaken ID publons researchmap
Ideguchi, Hidetaka Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Yoshimura-Nakagawa, Saki Department of Periodontics and Endodontics, Division of Dentistry, Okayama University Hospital
Sakaida, Kyosuke Department of Periodontics and Endodontics, Division of Dentistry, Okayama University Hospital
Nagata-Kamei, Chiaki Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kobayashi, Hiroya Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Ishii, Satoki Division of Applied Chemistry, Graduate School of Natural Sciences and Technology, Okayama University
Ono, Mitsuaki Department of Molecular Biology and Biochemistry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID researchmap
Ibaragi, Soichiro Department of Oral and Maxillofacial Surgery and Biopathology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
Yamamoto, Tadashi Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
Suga, Seiji Division of Applied Chemistry, Graduate School of Natural Sciences and Technology, Okayama University ORCID Kaken ID researchmap
Takashiba, Shogo Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap
抄録
Current periodontal treatment focuses on the mechanical removal of the source of infection, such as bacteria and their products, and there is no approach to control the host inflammatory response that leads to tissue destruction. In order to control periodontal inflammation, we have previously reported the optimization of (+)-terrein synthesis methods and the inhibitory effect of (+)-terrein on osteoclast differentiation in vitro. However, the pharmacological effect of (+)-terrein in vivo in the periodontitis model is still unknown. In this study, we investigated the effect of synthetic (+)-terrein on inflammatory bone resorption using a ligature-induced periodontitis mouse model. Synthetic (+)-terrein (30 mg/kg) was administered intraperitoneally twice a week to the mouse periodontitis model. The control group was treated with phosphate buffer. One to two weeks after the induction of periodontitis, the periodontal tissues were harvested for radiological evaluation (micro-CT), histological evaluation (HE staining and TRAP staining), and the evaluation of inflammatory cytokine production in the periodontal tissues and serum (quantitative reverse-transcription PCR, ELISA). The synthetic (+)-terrein-treated group suppressed alveolar bone resorption and the number of osteoclasts in the periodontal tissues compared to the control group (p < 0.05). In addition, synthetic (+)-terrein significantly suppressed both mRNA expression of TNF-α in the periodontal tissues and the serum concentration of TNF-α (both p < 0.05). In conclusion, we have demonstrated that synthetic (+)-terrein abrogates alveolar bone resorption via the suppression of TNF-α production and osteoclast differentiation in vivo. Therefore, we could expect potential clinical effects when using (+)-terrein on inflammatory bone resorption, including periodontitis.
キーワード
synthetic (+)-terrein
periodontitis
TNF-α
発行日
2023-03-03
出版物タイトル
Journal of Fungi
9巻
3号
出版者
MDPI
開始ページ
314
ISSN
2309-608X
資料タイプ
学術雑誌論文
言語
英語
OAI-PMH Set
岡山大学
著作権者
© 2023 by the authors.
論文のバージョン
publisher
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.3390/jof9030314
ライセンス
https://creativecommons.org/licenses/by/4.0/
Citation
Sako, H.; Omori, K.; Nakayama, M.; Mandai, H.; Ideguchi, H.; Yoshimura-Nakagawa, S.; Sakaida, K.; Nagata-Kamei, C.; Kobayashi, H.; Ishii, S.; et al. The Fungal Metabolite (+)-Terrein Abrogates Inflammatory Bone Resorption via the Suppression of TNF- Production in a Ligature-Induced Periodontitis Mouse Model. J. Fungi 2023, 9, 314. https://doi.org/10.3390/jof9030314
助成機関名
Japan Society for the Promotion of Science
助成番号
19K10108
21K16992