ID | 65157 |
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著者 |
Sako, Hidefumi
Department of Periodontics and Endodontics, Division of Dentistry, Okayama University Hospital
Omori, Kazuhiro
Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Nakayama, Masaaki
Department of Oral Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Mandai, Hiroki
Department of Pharmacy, Faculty of Pharmacy, Gifu University of Medical Science
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Ideguchi, Hidetaka
Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Yoshimura-Nakagawa, Saki
Department of Periodontics and Endodontics, Division of Dentistry, Okayama University Hospital
Sakaida, Kyosuke
Department of Periodontics and Endodontics, Division of Dentistry, Okayama University Hospital
Nagata-Kamei, Chiaki
Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kobayashi, Hiroya
Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Ishii, Satoki
Division of Applied Chemistry, Graduate School of Natural Sciences and Technology, Okayama University
Ono, Mitsuaki
Department of Molecular Biology and Biochemistry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Ibaragi, Soichiro
Department of Oral and Maxillofacial Surgery and Biopathology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Yamamoto, Tadashi
Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Suga, Seiji
Division of Applied Chemistry, Graduate School of Natural Sciences and Technology, Okayama University
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Takashiba, Shogo
Department of Pathophysiology-Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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抄録 | Current periodontal treatment focuses on the mechanical removal of the source of infection, such as bacteria and their products, and there is no approach to control the host inflammatory response that leads to tissue destruction. In order to control periodontal inflammation, we have previously reported the optimization of (+)-terrein synthesis methods and the inhibitory effect of (+)-terrein on osteoclast differentiation in vitro. However, the pharmacological effect of (+)-terrein in vivo in the periodontitis model is still unknown. In this study, we investigated the effect of synthetic (+)-terrein on inflammatory bone resorption using a ligature-induced periodontitis mouse model. Synthetic (+)-terrein (30 mg/kg) was administered intraperitoneally twice a week to the mouse periodontitis model. The control group was treated with phosphate buffer. One to two weeks after the induction of periodontitis, the periodontal tissues were harvested for radiological evaluation (micro-CT), histological evaluation (HE staining and TRAP staining), and the evaluation of inflammatory cytokine production in the periodontal tissues and serum (quantitative reverse-transcription PCR, ELISA). The synthetic (+)-terrein-treated group suppressed alveolar bone resorption and the number of osteoclasts in the periodontal tissues compared to the control group (p < 0.05). In addition, synthetic (+)-terrein significantly suppressed both mRNA expression of TNF-α in the periodontal tissues and the serum concentration of TNF-α (both p < 0.05). In conclusion, we have demonstrated that synthetic (+)-terrein abrogates alveolar bone resorption via the suppression of TNF-α production and osteoclast differentiation in vivo. Therefore, we could expect potential clinical effects when using (+)-terrein on inflammatory bone resorption, including periodontitis.
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キーワード | synthetic (+)-terrein
periodontitis
TNF-α
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発行日 | 2023-03-03
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出版物タイトル |
Journal of Fungi
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巻 | 9巻
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号 | 3号
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出版者 | MDPI
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開始ページ | 314
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ISSN | 2309-608X
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2023 by the authors.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.3390/jof9030314
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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Citation | Sako, H.; Omori, K.; Nakayama, M.; Mandai, H.; Ideguchi, H.; Yoshimura-Nakagawa, S.; Sakaida, K.; Nagata-Kamei, C.; Kobayashi, H.; Ishii, S.; et al. The Fungal Metabolite (+)-Terrein Abrogates Inflammatory Bone Resorption via the Suppression of TNF- Production in a Ligature-Induced Periodontitis Mouse Model. J. Fungi 2023, 9, 314. https://doi.org/10.3390/jof9030314
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助成機関名 |
Japan Society for the Promotion of Science
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助成番号 | 19K10108
21K16992
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