ID | 63266 |
フルテキストURL | |
著者 |
Xue, Ruizhi
Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Lin, Wenfeng
Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Fujita, Hirofumi
Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Sun, Jingkai
Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kinoshita, Rie
Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Ochiai, Kazuhiko
Laboratory of Veterinary Hygiene, Nippon Veterinary and Life Science University
Futami, Junichiro
Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
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Watanabe, Masami
Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Ohuchi, Hideyo
Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Sakaguchi, Masakiyo
Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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Tang, Zhengyan
Department of Urology, Xiangya Hospital, Central South University
Huang, Peng
Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Nasu, Yasutomo
Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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抄録 | The role of Dickkopf-3 (Dkk3)/REIC (The Reduced Expression in Immortalized Cells), a Wnt-signaling inhibitor, in male reproductive physiology remains unknown thus far. To explore the functional details of Dkk3/REIC in the male reproductive process, we studied the Dkk3/REIC knock-out (KO) mouse model. By examining testicular sections and investigating the sperm characteristics (count, vitality and motility) and ultrastructure, we compared the reproductive features between Dkk3/REIC-KO and wild-type (WT) male mice. To further explore the underlying molecular mechanism, we performed RNA sequencing (RNA-seq) analysis of testicular tissues. Our results showed that spermiation failure existed in seminiferous tubules of Dkk3/REIC-KO mice, and sperm from Dkk3/REIC-KO mice exhibited inferior motility (44.09 +/- 8.12% vs. 23.26 +/- 10.02%, p < 0.01). The Ultrastructure examination revealed defects in the sperm fibrous sheath of KO mice. Although the average count of Dkk3/REIC-KO epididymal sperm was less than that of the wild-types (9.30 +/- 0.69 vs. 8.27 +/- 0.87, x10(6)), neither the gap (p > 0.05) nor the difference in the sperm vitality rate (72.83 +/- 1.55% vs. 72.50 +/- 0.71%, p > 0.05) were statistically significant. The RNA-seq and GO (Gene Oncology) enrichment results indicated that the differential genes were significantly enriched in the GO terms of cytoskeleton function, cAMP signaling and calcium ion binding. Collectively, our research demonstrates that Dkk3/REIC is involved in the process of spermiation, fibrous sheath integrity maintenance and sperm motility of mice.
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キーワード | Dkk3/REIC
fibrous sheath
knock-out
RNA-seq
spermiation
sperm motility
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発行日 | 2022-01-31
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出版物タイトル |
Genes
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巻 | 13巻
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号 | 2号
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出版者 | MDPI
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開始ページ | 285
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ISSN | 2073-4425
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2022 by the authors.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.3390/genes13020285
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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Citation | Xue, R.; Lin, W.; Fujita, H.; Sun, J.; Kinoshita, R.; Ochiai, K.; Futami, J.; Watanabe, M.; Ohuchi, H.; Sakaguchi, M.; et al. Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice. Genes 2022, 13, 285. https://doi.org/10.3390/genes13020285
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助成機関名 |
Ministry of Education, Culture, Sports, Science and Technology
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助成番号 | 17K11138
21K09371
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