ID | 57932 |
フルテキストURL | |
著者 |
Apriliana Cahya Khayrani
Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
ORCID
Mahmud, Hafizah
Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
ORCID
publons
Zahra, Maram H.
Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
Aung Ko Ko Oo
Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
Oze, Miharu
Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
Du, Juan
Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
Alam, Md Jahangir
Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
Afify, Said M.
Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
ORCID
Hagar A. Abu Quora
Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Shigehiro, Tsukasa
Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
ORCID
Kaken ID
researchmap
Anna Sanchez Calle
Division of Molecular and Cellular Medicine, National Cancer Center Research Institute
Okada, Nobuhiro
Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Kaken ID
researchmap
Seno, Akimasa
Laboratory of Nano-Biotechnology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Fujita, Koki
Ensuiko Sugar Refining Co., Ltd.
Hamada, Hiroki
Faculty of Science, Okayama University of Science
Seno, Yuhki
Graduate School of Pharmaceutical Science, Tokushima University
Mandai, Tadakatsu
Faculty of Life Science, Kurashiki University of Science and the Arts
Seno, Masaharu
Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University
ORCID
Kaken ID
publons
researchmap
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抄録 | Paclitaxel (PTX) is one of the front-line drugs approved for the treatment of ovarian cancer. However, the application of PTX is limited due to the significant hydrophobicity and poor pharmacokinetics. We previously reported target-directed liposomes carrying tumor-selective conjugated antibody and encapsulated glycosylated PTX (gPTX-L) which successfully overcome the PTX limitation. The tubulin stabilizing activity of gPTX was equivalent to that of PTX while the cytotoxic activity of gPTX was reduced. In human ovarian cancer cell lines, SK-OV-3 and OVK18, the concentration at which cell growth was inhibited by 50% (IC50) for gPTX range from 15⁻20 nM, which was sensitive enough to address gPTX-L with tumor-selective antibody coupling for ovarian cancer therapy. The cell membrane receptor CD44 is associated with cancer progression and has been recognized as a cancer stem cell marker including ovarian cancer, becoming a suitable candidate to be targeted by gPTX-L therapy. In this study, gPTX-loading liposomes conjugated with anti-CD44 antibody (gPTX-IL) were assessed for the efficacy of targeting CD44-positive ovarian cancer cells. We successfully encapsulated gPTX into liposomes with the loading efficiency (LE) more than 80% in both of gPTX-L and gPTX-IL with a diameter of approximately 100 nm with efficacy of enhanced cytotoxicity in vitro and of convenient treatment in vivo. As the result, gPTX-IL efficiently suppressed tumor growth in vivo. Therefore gPTX-IL could be a promising formulation for effective ovarian cancer therapies.
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キーワード | CD44
glycosylated paclitaxel
liposome
modified paclitaxel
ovarian cancer
specific targeting
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発行日 | 2019-02-27
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出版物タイトル |
International Journal of Molecular Sciences
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巻 | 20巻
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号 | 5号
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出版者 | MDPI
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開始ページ | 1042
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ISSN | 1422-0067
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2019 by the authors.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.3390/ijms20051042
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ライセンス | http://creativecommons.org/licenses/by/4.0/
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助成機関名 |
日本学術振興会
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助成番号 | 25242045
26640079
JP18K15243
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