ID | 67518 |
フルテキストURL | |
著者 |
Kawase, Tomoka
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Debnath, Anusuya
Department of Biotechnology, Brainware University
Okamoto, Keinosuke
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Kaken ID
publons
researchmap
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抄録 | Background:Vibrio vulnificus NCIMB2137, a Gram-negative, metalloprotease negative estuarine strain was isolated from a diseased eel. A 45 kDa chymotrypsin-like alkaline serine protease known as VvsA has been recently reported as one of the major virulence factor responsible for the pathogenesis of this strain. The vvsA gene along with a downstream gene vvsB, whose function is still unknown constitute an operon designated as vvsAB. Objective: This study examines the contribution of VvsB to the functionality of VvsA. Method: In this study, VvsB was individually expressed using Rapid Translation System (RTS system), followed by an analysis of its role in regulating the serine protease activity of VvsA. Result: The proteolytic activity of VvsA increased upon the addition of purified VvsB to the culture supernatant of V. vulnificus. However, the attempts of protein expression using an E. coli system revealed a noteworthy observation that protein expression from the vvsA gene exhibited higher protease activity compared to that from the vvsAB gene within the cytoplasmic fraction. These findings suggest an intricate interplay between VvsB and VvsA, where VvsB potentially interacts with VvsA inside the bacterium and suppress the proteolytic activity. While outside the bacterial milieu, VvsB appears to stimulate the activation of inactive VvsA. Conclusion: The findings suggest that Vibrio vulnificus regulates VvsA activity through the action of VvsB, both intracellularly and extracellularly, to ensure its survival.
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キーワード | RTS system
in vitro cell-free translation system, PU
Proteinase unit, VvsA
Vibrio vulnificus serine protease, SD
Shine-Dalgarno sequence
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発行日 | 2024
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出版物タイトル |
FEMS Microbiology Letters
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巻 | 371巻
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出版者 | Oxford University Press (OUP)
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開始ページ | fnae053
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ISSN | 1574-6968
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © The Author(s) 2024.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
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関連URL | isVersionOf https://doi.org/10.1093/femsle/fnae053
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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Citation | Tomoka Kawase, Anusuya Debnath, Keinosuke Okamoto, Regulatory role of VvsB protein on serine protease activity of VvsA in Vibrio vulnificus, FEMS Microbiology Letters, Volume 371, 2024, fnae053, https://doi.org/10.1093/femsle/fnae053
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助成機関名 |
Ministry of Education, Culture, Sports, Science and Technology
Japan Agency for Medical Research and Development
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