ID | 30947 |
JaLCDOI | |
フルテキストURL | |
著者 |
Khan, Rasel
Laboratory of Protein Function, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Takahashi, Eizo
Laboratory of Protein Function, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
publons
researchmap
Nakura, Hironori
Laboratory of Protein Function, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
ORCID
Ansaruzzaman, Mohammad
Laboratory Sciences Division, ICDDR, B (International Centre for Diarrhoeal Diseases Research, Bangladesh), Centre for Health and Population Research
Banik, Sukalyani
Laboratory of Protein Function, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ramamurthy, Thandavarayan
Collaborative Research Center of Okayama University for Infectious Diseases in India
Okamoto, Keinosuke
Laboratory of Protein Function, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
publons
researchmap
|
抄録 | Aeromonas are water-borne pathogens. They are halotolerant, which means that they can survive in environments whose salt content corresponds to that of seawater (3.0% NaCl). However, the presence of Aeromonas in seawater is extremely rare compared with that in river water. In this study, we tested the ability of Aeromonas sobria to produce toxins in river water and seawater. First, we cultured A. sobria on skim milk agar plates supplemented with either river water (SARW) or seawater (SASW). The bacteria grew on both plates. A clear zone around the bacteria was generated in SARW. However, such a zone was not observed in SASW, suggesting that proteases were not generated in SASW. Subsequently, we cultured A. sobria in a nutrient broth supplemented with either river water (NRW) or with seawater (NSW), and examined the protease activity of their culture supernatants. The protease activity of the culture supernatant from NSW was extremely low compared to that from NRW. The immunoblotting analysis showed that serine protease (ASP) was not produced by the culture in NSW. By contrast, aerolysin-like hemolysin was produced in all conditions examined in this study. This indicates that the salinity of water is deeply involved in the production of ASP by A. sobria. |
キーワード | Aeromonas
water
toxin
salinity
|
Amo Type | Original Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 2008-12
|
巻 | 62巻
|
号 | 6号
|
出版者 | Okayama University Medical School
|
開始ページ | 363
|
終了ページ | 371
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
Web of Science KeyUT |