ID | 60849 |
フルテキストURL | |
著者 |
Debnath, Anusuya
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Mizuno, Tamaki
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
researchmap
Miyoshi, Shin-Ichi
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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抄録 | Vibrios can degrade chitin surfaces to soluble N-acetyl glucosamine oligosaccharides (GlcNAc(n)) that can be utilized as a carbon source and also induce a state of natural genetic competence. In this study, we characterized chitin-dependent growth and natural competence in Vibrio parahaemolyticus and its regulation. We found that growth on chitin was regulated through chitin sensors ChiS (sensor histidine kinase) and TfoS (transmembrane transcriptional regulator) by predominantly controlling the expression of chitinase VPA0055 (ChiA2) in a TfoX-dependent manner. The reduced growth of Delta chiA2, Delta chiS and Delta tfoS mutants highlighted the critical role played by ChiA2 in chitin breakdown. This growth defect of Delta chiA2 mutant could be recovered when chitin oligosaccharides GlcNAc(2) or GlcNAc(6) were supplied instead of chitin. The Delta tfoS mutant was also able to grow on GlcNAc(2) but the Delta chiS mutant could not, which indicates that GlcNAc(2) catabolic operon is dependent on ChiS and independent of TfoS. However, the Delta tfoS mutant was unable to utilize GlcNAc(6) because the periplasmic enzymes required for the breakdown of GlcNAc(6) were found to be downregulated at the mRNA level. We also showed that natural competence can be induced only by GlcNAc(6), not GlcNAc(2), because the expression of competence genes was significantly higher in the presence of GlcNAc(6) compared to GlcNAc(2). Moreover, this might be an indication that GlcNAc(2) and GlcNAc(6) were detected by different receptors. Therefore, we speculate that GlcNAc(2)-dependent activation of ChiS and GlcNAc(6)-dependent activation of TfoS might be crucial for the induction of natural competence in V. parahaemolyticus through the upregulation of the master competence regulator TfoX.
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キーワード | chitin
chitinase
GlcNAc(6)
natural competence
ChiA2
ChiS
TfoS
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発行日 | 2020-08-26
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出版物タイトル |
Microorganisms
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巻 | 8巻
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号 | 9号
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出版者 | MDPI
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開始ページ | 1303
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ISSN | 2076-2607
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2020 by the authors.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.3390/microorganisms8091303
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ライセンス | http://creativecommons.org/licenses/by/4.0/
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助成機関名 |
文部科学省
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助成番号 | JP19fm0108002
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