ID | 60595 |
フルテキストURL | |
著者 |
Matsuo, Toshihiko
Regenerative and Reconstructive Medicine (Ophthalmology), Okayama University Medical School and Graduate School of Interdisciplinary Science and Engineering in Health Systems
ORCID
Kaken ID
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Terada, Keiko
Research Center, Techno Pro, Inc.
Sakurai, Mikako
Research Center, Techno Pro, Inc.
Liu, Shihu
Regenerative and Reconstructive Medicine (Ophthalmology), Okayama University Medical School and Graduate School of Interdisciplinary Science and Engineering in Health Systems
Yamashita, Koichiro
Polymer Materials Science, Okayama University Faculty of Engineering and Graduate School of Natural Science and Technology
Uchida, Tetsuya
Polymer Materials Science, Okayama University Faculty of Engineering and Graduate School of Natural Science and Technology
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抄録 | Purpose: Multielectrode array recording for electric activity in cardiac and neuronal cells has been developed as preclinical tests for drug screening. This study aims to establish an in vitro assay system, using the multielectrode array, to record light-evoked spikes in isolated degenerative retinal tissues of retinal dystrophic rd1 mouse, as a preclinical test to examine the efficacy of photoelectric
dye-coupled thin film retinal prosthesis.
Methods: Light-evoked spike response was tested for 1 min at first step in the isolated degenerative retinal tissue of retinal dystrophic rd1 mouse only on the multielectrode array, tested in the same retinal tissue overlain with a plain control film for light-off and light-on 10 min each at second step, and tested in the same tissue overlain with a dye-coupled film at third step. The retinal tissues which showed light-evoked response at first or second step were not used for evaluation at third step.
Results: Residual light-evoked spikes were recorded at first or second step in 18 of 35 retinal tissues (51%) at 6 weeks of the age in rd1 mice, 16 of 44 tissues (36%) at 7 weeks, and 10 of 39 tissues (25%) at 8 weeks. At third step, light-evoked spikes were recorded with dye-coupled films in 8 of 17 retinal tissues (47%) at 6 weeks, 10 of 28 tissues (35%) at 7 weeks, and 8 of 29 tissues (27%) at 8 weeks.
Conclusion: A step-by-step procedure with internal control was established to measure light-evoked spikes by the multielectrode array in the isolated degenerative retinal tissue to evaluate photoelectric dye-coupled thin films. This preclinical study would present one line of evidence for the efficacy of photoelectric dye-coupled thin film retinal prosthesis towards a first-in-human clinical trial.
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キーワード | Photoelectric dye
Polyethylene thin film
Spike
Degenerative retina
Retinal dystrophic rd1 mouse
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発行日 | 2020-08-10
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出版物タイトル |
Clinics in Surgery
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巻 | 5巻
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出版者 | Remedy
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開始ページ | 2903
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ISSN | 2474-1647
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2020 Matsuo T.
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論文のバージョン | publisher
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オフィシャル URL | http://www.clinicsinsurgery.com/
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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