| ID | 56096 |
| フルテキストURL | |
| 著者 |
Uemura, Ryota
Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
Ogura, Mikako
Faculty of Agriculture , Okayama University
Matsumaru, Chihiro
Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
Akiyama, Tsuyoshi
Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
Maeda, Megumi
Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
Kaken ID
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Kimura, Yoshinobu
Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University
Kaken ID
publons
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| 抄録 | Acidic peptide:N-glycanase (aPNGase) plays a pivotal role in plant glycoprotein turnover. For the construction of aPNGase-knockout or -overexpressing plants, a new method to detect the activity in crude plant extracts is required because endogenous peptidases present in the extract hamper enzyme assays using fluorescence-labeled N-glycopeptides as a substrate. In this study, we developed a new method for measuring aPNGase activity in crude extracts from plant materials
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| キーワード | Acidic PNGase
FNG: free N-glycan
Fuc: L-fucose
Gal: D-galactose
GlcNAc: N-acetyl-D-glucosamine
HPLC: high-performance liquid chromatography
Man: D-mannose
NeuNAc2Gal2GlcNAc2Man3GlcNAc1: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAc
NeuNAc2Gal2GlcNAc2Man3GlcNAc2: NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–6(NeuNAcα2–6Galβ1–4GlcNAcβ1–2Manα1–3)Manβ1–4GlcNAcβ1–4GlcNAc
NeuNAc: N-acetylneuraminic acid
PA-: pyridylamino
PNGase-A: aPNGase from almond seed
PNGase-Le: aPNGase from tomato (Solanum lycopersium L.)
PNGase: peptide:N-glycanase
PTC: plant complex type
RCA120: Ricinus communis agglutinin (120 kDa)
RP-HPLC: reversed-phase HPLC
SF-HPLC: size-fractionation HPLC
Xyl: D-xylose
affinity chromatography
enzyme assay
free N-glycans
transgenic plant
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| 発行日 | 2018-04
|
| 出版物タイトル |
Bioscience, Biotechnology, and Biochemistry
|
| 巻 | 82巻
|
| 号 | 7号
|
| 出版者 | Taylor & Francis
|
| 開始ページ | 1172
|
| 終了ページ | 1175
|
| ISSN | 09168451
|
| NCID | AA10824164
|
| 資料タイプ |
学術雑誌論文
|
| 言語 |
英語
|
| OAI-PMH Set |
岡山大学
|
| 論文のバージョン | author
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| PubMed ID | |
| DOI | |
| Web of Science KeyUT | |
| 関連URL | isVersionOf https://doi.org/10.1080/09168451.2018.1459464
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| 助成機関名 |
文部科学省
|
| 助成番号 | 15K07841
15KK0282
17K0819709
|
