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ID 31626
JaLCDOI
フルテキストURL
著者
Matsushita, Akio Okayama University
Tabata, Masahiro Okayama University
Ueoka, Hiroshi Okayama University
Kiura, Katsuyuki Okayama University
Shibayama, Takuo Okayama University
Aoe, Keisuke Okayama University
Kohara, Hiroyuki Okayama University
Harada, Mine Okayama University
抄録

We established a drug sensitivity panel consisting of 24 human lung cancer cell lines. Using this panel, we evaluated 26 anti-cancer agents: three alkylators, three platinum compounds, four antimetabolites, one topoisomerase I inhibitor, five topoisomerase II inhibitors, seven antimitotic agents and three tyrosine kinase inhibitors. This panel showed the following: a) Drug sensitivity patterns reflected their clinically-established patterns of action. For example, doxorubicin and etoposide were shown to be active against small cell lung cancer cell lines and mitomycin-C and 5-fluorouracil were active against non-small cell lung cancer cell lines, in agreement with clinical data. b) Correlation analysis of the mean graphs derived from the logarithm of IC50 values of the drugs gave insight into the mechanism of each drug's action. Thus, two drug combinations with reverse or no correlation, such as the combination of cisplatin and vinorelbine, might be good candidates for the ideal two drug combination in the treatment of lung cancer, as is being confirmed in clinical trials. c) Using cluster analysis of the cell lines in the panel with their drug sensitivity patterns, we could classify the cell lines into four groups depending on the drug sensitivity similarity. This classification will be useful to elucidate the cellular mechanism of action and drug resistance. Thus, our drug sensitivity panel will be helpful to explore new drugs or to develop a new combination of anti-cancer agents for the treatment of lung cancer.

キーワード
drug screening system
MTT assay
lung cancer cell line
drug resistance
Amo Type
Article
発行日
1999-04
出版物タイトル
Acta Medica Okayama
53巻
2号
出版者
Okayama University Medical School
開始ページ
67
終了ページ
75
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
Web of Sience KeyUT