ID | 50974 |
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著者 |
Tabara, Ayumi
Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
Kaneta, Takashi
Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
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抄録 | Here, we report a novel method consisting of capillary electrophoretic separation followed by two-color LIF detection with postcolumn derivatization. The method can be used to discriminate glycoproteins in a protein mixture containing both glycosylated and unglycosylated proteins. The detector permitted simultaneous measurements of two electropherograms obtained by 450 nm (diode laser) and 532 nm (Nd:YAG laser) lasers excited native proteins following postcolumn derivatization with naphthalene-2,3-dicarboxaldehyde and concanavalin A (Con A) labeled with tetramethylrhodamine (rhodamine-labeled Con A), respectively. So, a protein can be assigned as glycosylated if it shows a peak at the same migration time in both electropherograms. According to the proposed principle, in a single run we discriminated a glycosylated protein (thyroglobulin) from an unglycosylated protein (albumin) in the presence of rhodamine-labeled Con A. Because the methodology permits the simultaneous detection of native proteins and their complexes with a fluorescently labeled probe, it should have broad applicability to binding assays.
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キーワード | Capillary electrophoresis
Concanavalin A
Glycoprotein
Postcolumn derivatization
Two-color laser-induced fluorescence
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発行日 | 2013-08
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出版物タイトル |
Electrophoresis
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巻 | 34巻
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号 | 16号
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出版者 | WILEY-VCH
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開始ページ | 2316
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終了ページ | 2322
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ISSN | 01730835
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NCID | AA00179882
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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論文のバージョン | author
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DOI | |
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関連URL | isVersionOf https://doi.org/10.1002/elps.201300149
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