ID | 59923 |
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著者 |
Kondo, Hideki
Institute of Plant Science and Resources (IPSR), Okayama University
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Fujita, Miki
Institute of Plant Science and Resources (IPSR), Okayama University
Hisano, Hiroshi
Institute of Plant Science and Resources (IPSR), Okayama University
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Hyodo, Kiwamu
Institute of Plant Science and Resources (IPSR), Okayama University
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Andika, Ida Bagus
College of Plant Health and Medicine, Qingdao Agricultural University
Suzuki, Nobuhiro
Institute of Plant Science and Resources (IPSR), Okayama University
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抄録 | Aphids (order Hemiptera) are important insect pests of crops and are also vectors of many plant viruses. However, little is known about aphid-infecting viruses, particularly their diversity and relationship to plant viruses. To investigate the aphid viromes, we performed deep sequencing analyses of the aphid transcriptomes from infested barley plants in a field in Japan. We discovered virus-like sequences related to nege/kita-, flavi-, tombus-, phenui-, mononega-, narna-, chryso-, partiti-, and luteoviruses. Using RT-PCR and sequence analyses, we determined almost complete sequences of seven nege/kitavirus-like virus genomes; one of which was a variant of the Wuhan house centipede virus (WHCV-1). The other six seem to belong to four novel viruses distantly related to Wuhan insect virus 9 (WhIV-9) or Hubei nege-like virus 4 (HVLV-4). We designated the four viruses as barley aphid RNA virus 1 to 4 (BARV-1 to -4). Moreover, some nege/kitavirus-like sequences were found by searches on the transcriptome shotgun assembly (TSA) libraries of arthropods and plants. Phylogenetic analyses showed that BARV-1 forms a clade with WHCV-1 and HVLV-4, whereas BARV-2 to -4 clustered with WhIV-9 and an aphid virus, Aphis glycines virus 3. Both virus groups (tentatively designated as Centivirus and Aphiglyvirus, respectively), together with arthropod virus-like TSAs, fill the phylogenetic gaps between the negeviruses and kitaviruses lineages. We also characterized the flavi/jingmen-like and tombus-like virus sequences as well as other RNA viruses, including six putative novel viruses, designated as barley aphid RNA viruses 5 to 10. Interestingly, we also discovered that some aphid-associated viruses, including nege/kita-like viruses, were present in different aphid species, raising a speculation that these viruses might be distributed across different aphid species with plants being the reservoirs. This study provides novel information on the diversity and spread of nege/kitavirus-related viruses and other RNA viruses that are associated with aphids.
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キーワード | negevirus
kitavirus
aphid
virome
RNA seq
barley
diversity
horizontal transmission
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発行日 | 2020-04-07
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出版物タイトル |
Frontiers in Microbiology
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巻 | 11巻
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出版者 | Frontiers Media
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開始ページ | 509
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ISSN | 1664-302X
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2020 Kondo, Fujita, Hisano, Hyodo, Andika and Suzuki.
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論文のバージョン | publisher
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関連URL | isVersionOf https://doi.org/10.3389/fmicb.2020.00509
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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