ID | 49558 |
フルテキストURL | |
著者 |
Kosaka, H
Ichikawa, T
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Kaken ID
publons
researchmap
Kambara, H
Inoue, S
Maruo, T
Nakamura, K
Hokkaido Univ, Grad Sch Life Sci, Fac Adv Life Sci, Innate Immun Lab
Hamada, H
Tokyo Univ Pharm & Life Sci, Dept Life Sci
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抄録 | We evaluated a new therapeutic strategy for malignant glioma, which combines intratumoral inoculation of mesenchymal stem cells (MSCs) expressing cytosine deaminase gene with 5-fluorocytosine (5-FC) administration. For in vitro and in vivo experiments, MSCs were transfected with adenovirus carrying either enhanced green fluorescent protein gene (AdexCAEGFP) or cytosine deaminase gene (AdexCACD), to establish MSC-expressing EGFP (MSC-EGFP) or CD (MSC-CD). Co-culture of 9L glioma cells with MSC-CD in a medium containing 5-FC resulted in a remarkable reduction in 9L cell viability. The migratory ability of MSC-EGFP toward 9L cells was demonstrated by double-chamber assay. For the in vivo study, rats harboring 9L brain tumors were inoculated with MSC-EGFP or MSC-CD. Immunohistochemistry of rat brain tumors inoculated with MSC-EGFP showed intratumoral distribution of MSC-EGFP. Survival analysis of rats bearing 9L gliomas treated with intratumoral MSC-CD and intraperitoneal 5-FC resulted in significant prolongation of survival compared with control animals. In conclusion, molecular therapy combining suicide gene therapy and MSCs as a targeting vehicle represents a potential new therapeutic approach for malignant glioma, both with respect to the antitumor potential of this system and its neuroprotective effect on normal brain tissue.
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キーワード | glioma
mesenchymal stem cell
suicide gene
bystander effect
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発行日 | 2012-08
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出版物タイトル |
Cancer Gene Therapy
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巻 | 19巻
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号 | 8号
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出版者 | Nature Publishing Group
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開始ページ | 572
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終了ページ | 578
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ISSN | 0929-1903
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NCID | AA12570566
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://dx.doi.org/10.1038/cgt.2012.35
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関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/49136
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言語 |
英語
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著作権者 | © Nature Publishing Group
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論文のバージョン | author
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査読 |
有り
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DOI | |
Web of Science KeyUT |