ID | 66943 |
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著者 |
Matsushima, Ryo
Institute of Plant Science and Resources, Okayama University
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Hisano, Hiroshi
Institute of Plant Science and Resources, Okayama University
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Galis, Ivan
Institute of Plant Science and Resources, Okayama University
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Miura, Satoko
Department of Biological Production, Akita Prefectural University
Crofts, Naoko
Department of Biological Production, Akita Prefectural University
Takenaka, Yuto
College of Life Sciences, Ritsumeikan University
Oitome, Naoko F.
Department of Biological Production, Akita Prefectural University
Ishimizu, Takeshi
College of Life Sciences, Ritsumeikan University
Fujita, Naoko
Department of Biological Production, Akita Prefectural University
Sato, Kazuhiro
Institute of Plant Science and Resources, Okayama University
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抄録 | Starch is a biologically and commercially important glucose polymer synthesized by plants as semicrystalline starch granules (SGs). Because SG morphology affects starch properties, mutants with altered SG morphology may be useful in breeding crops with desirable starch properties, including potentially novel properties. In this study, we employed a simple screen for mutants with altered SG morphology in barley (Hordeum vulgare). We isolated mutants that formed compound SGs together with the normal simple SGs in the endosperm and found that they were allelic mutants of the starch biosynthesis genes ISOAMYLASE1 (HvISA1) and FLOURY ENDOSPERM 6 (HvFLO6), encoding starch debranching enzyme and CARBOHYDRATE-BINDING MODULE 48-containing protein, respectively. We generated the hvflo6 hvisa1 double mutant and showed that it had significantly reduced starch biosynthesis and developed shrunken grains. In contrast to starch, soluble α-glucan, phytoglycogen, and sugars accumulated to higher levels in the double mutant than in the single mutants. In addition, the double mutants showed defects in SG morphology in the endosperm and in the pollen. This novel genetic interaction suggests that hvflo6 acts as an enhancer of the sugary phenotype caused by hvisa1 mutation.
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備考 | The version of record of this article, first published in Theoretical and Applied Genetics, is available online at Publisher’s website: http://dx.doi.org/10.1007/s00122-023-04339-5
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発行日 | 2023-04
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出版物タイトル |
Theoretical and Applied Genetics
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巻 | 136巻
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号 | 4号
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出版者 | Springer Science and Business Media LLC
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開始ページ | 94
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ISSN | 0040-5752
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © The Author(s) 2023
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
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関連URL | isVersionOf https://doi.org/10.1007/s00122-023-04339-5
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ライセンス | http://creativecommons.org/licenses/by/4.0/
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Citation | Matsushima, R., Hisano, H., Galis, I. et al. FLOURY ENDOSPERM 6 mutations enhance the sugary phenotype caused by the loss of ISOAMYLASE1 in barley. Theor Appl Genet 136, 94 (2023). https://doi.org/10.1007/s00122-023-04339-5
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助成機関名 |
Okayama University
New Energy and Industrial Technology Development Organization
TOYO INSTITUTE of FOOD TECHNOLOGY
Public Foundation of Elizabeth Arnold-Fuji
Takahashi Industrial and Economic Research Foundation
MAYEKAWA HOUONKAI FOUNDATION
G-7 Scholarship Foundation
Ohara Foundation
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助成番号 | 20K05970
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