ID | 64237 |
フルテキストURL | |
著者 |
Kinuhata, Toshiki
Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Sato, Keita
Department of Cytology and Histology, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
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Bando, Tetsuya
Department of Cytology and Histology, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
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Mito, Taro
Bio-Innovation Research Center, Tokushima University
Miyaishi, Satoru
Department of Legal Medicine, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
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Nohno, Tsutomu
Department of Cytology and Histology, Okayama University Medical School
Ohuchi, Hideyo
Department of Cytology and Histology, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences
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抄録 | The first event of differentiation and morphogenesis in the optic vesicle (OV) is specification of the neural retina (NR) and retinal pigment epithelium (RPE), separating the inner and outer layers of the optic cup, respectively. Here, we focus on a basic helix-loop-helix gene, BHLHE40, which has been shown to be expressed by the developing RPE in mice and zebrafish. Firstly, we examined the expression pattern of BHLHE40 in the developing chicken eye primordia by in situ hybridization. Secondly, BHLHE40 overexpression was performed with in ovo electroporation and its effects on optic cup morphology and expression of NR and RPE marker genes were examined. Thirdly, we examined the expression pattern of BHLHE40 in LHX1-overexpressed optic cup. BHLHE40 expression emerged in a subset of cells of the OV at Hamburger and Hamilton stage 14 and became confined to the outer layer of the OV and the ciliary marginal zone of the retina by stage 17. BHLHE40 overexpression in the prospective NR resulted in ectopic induction of OTX2 and repression of VSX2. Conversely, BHLHE40 was repressed in the second NR after LHX1 overexpression. These results suggest that emergence of BHLHE40 expression in the OV is involved in initial RPE specification and that BHLHE40 plays a role in separation of the early OV domains by maintaining OTX2 expression and antagonizing an NR developmental program.
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キーワード | basic helix-loop-helix e40
BHLHE40
LIM homeobox 1
LHX1
chicken
optic vesicle
retinal pigment epithelium
RPE
neural retina
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発行日 | 2022-10-29
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出版物タイトル |
Journal Of Developmental Biology
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巻 | 10巻
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号 | 4号
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出版者 | MDPI
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開始ページ | 45
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ISSN | 2221-3759
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2022 by the authors.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.3390/jdb10040045
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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