ID | 50710 |
フルテキストURL | |
著者 |
Tanaka, Norimitsu
Okayama Univ, Dept Canc & Thorac Surg, Grad Sch Med Dent & Pharmaceut Sci
Toyooka, Shinichi
Okayama Univ, Dept Canc & Thorac Surg, Grad Sch Med Dent & Pharmaceut Sci
ORCID
Kaken ID
publons
researchmap
Soh, Junichi
Okayama Univ, Dept Canc & Thorac Surg, Grad Sch Med Dent & Pharmaceut Sci
ORCID
Kaken ID
researchmap
Kubo, Takafumi
Okayama Univ, Dept Canc & Thorac Surg, Grad Sch Med Dent & Pharmaceut Sci
Yamamoto, Hiromasa
Okayama Univ, Dept Canc & Thorac Surg, Grad Sch Med Dent & Pharmaceut Sci
ORCID
Kaken ID
publons
researchmap
Maki, Yuho
Okayama Univ, Dept Canc & Thorac Surg, Grad Sch Med Dent & Pharmaceut Sci
Muraoka, Takayuki
Okayama Univ, Dept Canc & Thorac Surg, Grad Sch Med Dent & Pharmaceut Sci
Shien, Kazuhiko
Okayama Univ, Dept Canc & Thorac Surg, Grad Sch Med Dent & Pharmaceut Sci
ORCID
Kaken ID
publons
researchmap
Furukawa, Masashi
Okayama Univ, Dept Canc & Thorac Surg, Grad Sch Med Dent & Pharmaceut Sci
Ueno, Tsuyoshi
Okayama Univ, Dept Canc & Thorac Surg, Grad Sch Med Dent & Pharmaceut Sci
Asano, Hiroaki
Okayama Univ, Dept Canc & Thorac Surg, Grad Sch Med Dent & Pharmaceut Sci
Kaken ID
Tsukuda, Kazunori
Okayama Univ, Dept Canc & Thorac Surg, Grad Sch Med Dent & Pharmaceut Sci
ORCID
Kaken ID
publons
researchmap
Aoe, Keisuke
Natl Hosp Org Yamaguchi Ube Med Ctr, Dept Med Oncol
Miyoshi, Shinichiro
Okayama Univ, Dept Canc & Thorac Surg, Grad Sch Med Dent & Pharmaceut Sci
Kaken ID
publons
researchmap
|
抄録 | Small-cell lung cancer (SCLC) is an aggressive tumor with a dismal prognosis among primary lung cancers. MicroRNAs (miRNAs) can act as oncogenes or tumor-suppressor genes in human malignancy. The miR-34 family is comprised of tumor-suppressive miRNAs, and its reduced expression by methylation has been reported in various cancers, including non-small cell lung cancer (NSCLC). In this study, we investigated the alteration and tumor-suppressive impact of miR-34s in SCLC. The methylation of miR-34a and miR-34b/c was observed in 4 (36%) and 7 (64%) of 11 SCLC cell lines, respectively. Among the 27 SCLC clinical specimens, miR-34a and miR-34b/c were methylated in 4(15%) and 18 (67%), respectively. In contrast, 13 (28%) miR-34a methylated cases and 12 (26%) miR-34b/c methylated cases were found in 47 NSCLC primary tumors. The frequency of miR-34b/c methylation was significantly higher in SCLC than in NSCLC (p < 0.001). The expressions of miR-34s were reduced in methylated cell lines and tumors and restored after 5-aza-2'-deoxycytidine treatment, indicating that methylation was responsible for the reduced expression of miR-34s. Because the frequency of methylation was higher in miR-34b/c, we focused on miR-34b/c for a functional analysis. We examined the effect of miR-34b/c introduction on cell proliferation, migration and invasion. The transfection of miR-34b/c to two SCLC cell lines (H1048 and SBC5) resulted in the significant inhibition of cell growth, migration, and invasion, compared with control transfectants. Our results indicate that the aberrant methylation of miR-34b/c plays an important role in the pathogenesis of SCLC, implying that miR-34b/c may be a useful therapeutic target for SCLC.
|
キーワード | Methylation
MicroRNA
MicroRNA-34b/c
Small cell lung cancer
Non-small cell lung cancer
p53
|
発行日 | 2012-04
|
出版物タイトル |
Lung Cancer
|
巻 | 76巻
|
号 | 1号
|
出版者 | Elsevier Ireland Ltd.
|
開始ページ | 32
|
終了ページ | 38
|
ISSN | 0169-5002
|
NCID | AA10785743
|
資料タイプ |
学術雑誌論文
|
オフィシャル URL | http://dx.doi.org/10.1016/j.lungcan.2011.10.002
|
関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/50698
|
言語 |
英語
|
著作権者 | (C) 2011 Elsevier Ireland Ltd. All rights reserved.
|
論文のバージョン | author
|
査読 |
有り
|
DOI | |
Web of Science KeyUT |