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ID 50625
フルテキストURL
著者
Maruo, Tomoko Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Ichikawa, Tomotsugu Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Kanzaki, Hirotaka Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Mol Genet
Inoue, Satoshi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Kurozumi, Kazuhiko Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Onishi, Manabu Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Yoshida, Koichi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Kambara, Hirokazu Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Ouchida, Mamoru Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Mol Genet 科研費研究者番号
Shimizu, Kenji Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Mol Genet
Tamaru, Seiji Okayama Univ, Sch Med, Cent Res Lab
Chiocca, E. Antonio Brigham & Womens Faulkner Hosp, Dept Neurosurg
Date, Isao Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg ORCID 科研費研究者番号 publons
抄録
One of the insidious biological features of gliomas is their potential to extensively invade normal brain tissue, yet molecular mechanisms that dictate this locally invasive behavior remain poorly understood. To investigate the molecular basis of invasion by malignant gliomas, proteomic analysis was performed using a pair of canine glioma subclones - J3T-1 and J3T-2 - that show different invasion phenotypes in rat brains but have similar genetic backgrounds. Two-dimensional protein electrophoresis of whole-cell lysates of J3T-1 (angiogenesis-dependent invasion phenotype) and J3T-2 (angiogenesis-independent invasion phenotype) was performed. Twenty-two distinct spots were recognized when significant alteration was defined as more than 1.5-fold change in spot intensity between J3T-1 and J3T-2. Four proteins that demonstrated increased expression in J3T-1, and 14 proteins that demonstrated increased expression in J3T-2 were identified using liquid chromatography-mass spectrometry analysis. One of the proteins identified was annexin A2, which was expressed at higher levels in J3T-1 than in J3T-2. The higher expression of annexin A2 in J3T-1 was corroborated by quantitative RT-PCR of the cultured cells and immunohistochemical staining of the rat brain tumors. Moreover, immunohistochemical analysis of human glioblastoma specimens showed that annexin A2 was expressed at high levels in the tumor cells that formed clusters around dilated vessels. These results reveal differences in the proteomic profiles between these two cell lines that might correlate with their different invasion profiles. Thus, annexin A2 may be related to angiogenesis-dependent invasion.
キーワード
angiogenesis
annexin A2
glioma
invasion
proteomics
発行日
2013-06
出版物タイトル
Neuropathology
33巻
3号
出版者
Wiley-Blackwell
開始ページ
264
終了ページ
275
ISSN
0919-6544
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1111/j.1440-1789.2012.01361.x
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/50652
言語
English
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT