ID | 52021 |
フルテキストURL | |
著者 |
Saito, Shunsuke
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Gastroenterol & Hepatol
Kato, Jun
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Gastroenterol & Hepatol
Hiraoka, Sakiko
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Gastroenterol & Hepatol
Kaken ID
publons
researchmap
Horii, Joichiro
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Gastroenterol & Hepatol
Suzuki, Hideyuki
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Gastroenterol & Hepatol
Higashi, Reiji
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Gastroenterol & Hepatol
Kaji, Eisuke
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Gastroenterol & Hepatol
Kondo, Yoshitaka
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Gastroenterol Surg Transplant & Surg Oncol
ORCID
Kaken ID
researchmap
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抄録 | Background: Although DNA methylation of colonic mucosa in ulcerative colitis (UC) has been suggested, the majority of published reports indicate the correlation between methylation of colon mucosa and occurrence of UC-related dysplasia or cancer without considering the mucosal inflammatory status. The aim of this study was to verify whether mucosal inflammation-specific DNA methylation occurs in the colon of UC.
Methods: Of 15 gene loci initially screened, six loci (ABCB1, CDH1. ESR1, GDNF, HPP1, and MYOD1) methylated in colon mucosa of UC were analyzed according to inflammatory status using samples from 28 surgically resected UC patients.
Results: Four of six regions (CDH1, GDNF, HPP1, and MYOD1) were more highly methylated in the active inflamed mucosa than in the quiescent mucosa in each UC patient (P = 0.003, 0.0002, 0.02, and 0.048, respectively). In addition, when the methylation status of all samples taken from examined patients was stratified according to inflammatory status, methylation of CDHI and GDNF loci was significantly higher in active inflamed mucosa than in quiescent mucosa (P = 0.045 and 0.002, respectively). Multiple linear regression analysis revealed that active inflammation was an independent factor of methylation for CDHI and GDNF. DNA methyltransferase 1 and 3b were highly expressed in colon epithelial cells with active mucosa] inflammation, suggesting their involvement in inflammation-dependent methylation.
Conclusions: Methylation in colonic mucosa of UC was correlated with mucosal inflammatory status, suggesting the involvement of methylation due to chronic active inflammation in UC carcinogenesis.
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キーワード | ulcerative colitis
inflammation
methylation
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備考 | This is a preprint of an article published in http://dx.doi.org/10.1002/ibd.21573
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発行日 | 2011-09
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出版物タイトル |
Inflammatory Bowel Diseases
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巻 | 17巻
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号 | 9号
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開始ページ | 1955
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終了ページ | 1965
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ISSN | 1078-0998
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://dx.doi.org/10.1002/ibd.21573
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関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/51952
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言語 |
英語
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論文のバージョン | author
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査読 |
有り
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DOI | |
Web of Science KeyUT |