| ID | 10573 |
| Eprint ID | 10573
|
| フルテキストURL | |
| タイトル（別表記） | 逆転写酵素・DNAポリメラーゼを用いたRT-PCRによる増幅反応で確認されたペッパーマイルドモットルウイルス（PMMoV）感染ピーマンの果実種子におけるウイルスの効果的な不活性化方法
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| 著者 |
Hikichi, Yasufumi
Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University
Takeuchi, Shigeharu
Laboratory of Plant Pathology, Kochi Agricultural Research Center
Okumura, Ako
Laboratory of Plant Pathology, Iwate Biotechnology Research Center (IBRC)
Nasu, Yoshiko
Laboratory of Plant Pathology, Iwate Biotechnology Research Center (IBRC)
Okuno, Tetsuro
Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
Suzuki, Kazumi
Laboratory of Plant Pathology, Iwate Biotechnology Research Center (IBRC)
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| 抄録 | A method for efficient inactivation Papper mild mottle virus (PMMoV) in harvested seeds of green pepper was examined based on the infectivity on the leaves of Nicotina glutinosa L.(a local host) and a reverse transcription and polymerase chain reaction (RT-PCR)-based amplification of the viral RNA. The seed homogenates from PMMoV-infected plants produced a large number of necrotic local lesion in N.glutinosa, but soaking the seeds in 10% (w/v) tri-sodium phosphate (Na3PO4) for 20 min or dry sterilization (70C,3h) effectivity eliminated the abundance of PMMoV. However, no necrotic lesion on N.glutinosa or the RT-PCR-basaed amplification was observed with seeds that has been disinfected by Na3PO4 in combination with dry sterilization.
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| キーワード | Capsicum annuum L.
pepper mild mottle virus (PMMoV)
RT-PCR
seed disinfection
seed transmission
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| 発行日 | 2004-02
|
| 出版物タイトル |
岡山大学農学部学術報告
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| 出版物タイトル（別表記） | Scientific reports of the Faculty of Agriculture, Okayama University
|
| 巻 | 93巻
|
| 号 | 1号
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| 出版者 | 岡山大学農学部
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| 出版者（別表記） | Faculty of Agriculture,Okayama University
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| 開始ページ | 29
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| 終了ページ | 32
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| ISSN | 0474-0254
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| NCID | AN00033029
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| 資料タイプ |
紀要論文
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| 言語 |
英語
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| 論文のバージョン | publisher
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| 査読 |
無し
|
| Eprints Journal Name | srfa
|
