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ID 33000
フルテキストURL
著者
Toeda, Kenichi Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Nakamura, Keigo Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Hirohata, Satoshi Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Hatipoglu, Omer F. Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine and Dentistry
Demircan, Kadir Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine and Dentistry
Yamawaki, Hitoshi Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Ogawa, Hiroko Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Kusachi, Shozo Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Shiratori, Yasushi Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry
Ninomiya, Yoshifumi Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine and Dentistry
抄録
Versican, a large chondroitin sulfate proteoglycan, plays a role in conditions such as wound healing and tissue remodelling. To test the hypothesis that versican expression is transiently upregulated and plays a role in the infarcted heart, we examined its expression in a rat model of myocardial infarction. Northern blot analysis demonstrated increased expression of versican mRNA. Quantitative real-time RT-PCR analysis revealed that versican mRNA began to increase as early as 6 h and reached its maximal level 2 days after coronary artery ligation. Versican mRNA then gradually decreased, while the mRNA of decorin, another small proteoglycan, increased thereafter. Versican mRNA was localized in monocytes, as indicated by CD68-positive staining, around the infarct tissue. The induction of versican mRNA was accelerated by ischemia/reperfusion (I/R), which was characterized by massive cell infiltration and enhanced inflammatory response. To examine the alteration of versican expression in monocytes/macrophages, we isolated human peripheral blood mononuclear cells and stimulated them with granulocyte/macrophage colony-stimulating factor (GM-CSF). Stimulation of mononuclear cells with GM-CSF increased the expression of versican mRNA as well as cytokine induction. The production of versican by monocytes in the infarct area represents a novel finding of the expression of an extracellular matrix gene by monocytes in the infarcted heart. We suggest that upregulation of versican in the infarcted myocardium may have a role in the inflammatory reaction, which mediates subsequent chemotaxis in the infarcted heart.
キーワード
coronary artery disease
cytokine
extracellular matrix
GM-CSF
monocyte
備考
Digital Object Identifer:10.1007/s11010-005-8051-4
Published with permission from the copyright holder. This is the author's copy, as published in Molecular and Cellular Biochemistry, December 2005, Volume 280, Issue 1-2, Pages 47-56.
Publisher URL:http://dx.doi.org/10.11997 The Roya007/s11010-005-8051-4
Direct access to Thomson Web of Science record
Copyright © 2005 Springer. All rights reserved.
発行日
2005-12
出版物タイトル
Molecular and Cellular Biochemistry
280巻
1-2号
出版者
Springer
開始ページ
47
終了ページ
56
ISSN
0300-8177
NCID
AA00745800
資料タイプ
学術雑誌論文
言語
English
著作権者
Springer
論文のバージョン
author
査読
有り
DOI
PubMed ID
Web of Sience KeyUT
Submission Path
biology_general/34