ID | 48980 |
フルテキストURL | |
著者 |
Matsuura, Koji
Research Core for Interdisciplinary Sciences, Okayama University
Kaken ID
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Kuroda, Yuka
Research Core for Interdisciplinary Sciences, Okayama University
Yamashita, Keisuke
Department of Animal Science, Faculty of Agriculture, Okayama University
Funahashi, Hiroaki
Department of Animal Science, Faculty of Agriculture, Okayama University
ORCID
Kaken ID
publons
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抄録 | Motile porcine sperms adhere to hydrophilic materials such as glass and plastics. The adsorption of sperms to a hydrophobic poly(dimethylsiloxane) (PDMS) membrane is less compared with that to glass. We investigated the linear velocity (LV) and amplitude of lateral head displacement (ALHD) of motile porcine sperm on glass and PDMS preparations using computer-assisted sperm analysis (CASA). Significant decreases were observed in the 15-min LV (P<0.05) and ALHD (P<0.05) in motile porcine sperm on glass preparations compared with those on PDMS preparations. These differences were due to adsorption of the head and/or neck to hydrophilic substrates. Because of the elasticity of PDMS, we propose that a PDMS membrane should be used for CASA. To investigate the dynamics of motile porcine sperms with microfluidics, we do not recommend plasma treatment to bond PDMS and glass in the microchannel preparation; instead, we suggest that a PDMS molding process without plasma treatment be used for preparation of microfluidic channels.
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キーワード | Adsorption
Porcine sperm motility
Silicone elastomer
Trajectories
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発行日 | 2010-10-12
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出版物タイトル |
Journal of Reproduction and Development
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巻 | 57巻
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号 | 1号
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出版者 | Society for Reproduction and Development
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開始ページ | 163
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終了ページ | 167
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ISSN | 0916-8818
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資料タイプ |
学術雑誌論文
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プロジェクト |
異分野融合先端研究コア
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オフィシャル URL | https://www.jstage.jst.go.jp/article/jrd/57/1/57_10-075N/_article
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言語 |
英語
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著作権者 | Copyright © 2011 Society for Reproduction and Development
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論文のバージョン | author
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査読 |
有り
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