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ID 49033
フルテキストURL
著者
Matsuo, Noriyuki Okayama Univ
Shiraha, Hidenori Okayama Univ
Fujikawa, Tatsuya Okayama Univ
Takaoka, Nobuyuki Okayama Univ
Ueda, Naoki Okayama Univ
Tanaka, Shigetomi Okayama Univ
Nishina, Shinichi Okayama Univ
Nakanishi, Yutaka Okayama Univ
Uemura, Masayuki Okayama Univ
Takaki, Akinobu Okayama Univ
Nakamura, Shinichiro Okayama Univ
Kobayashi, Yoshiyuki Okayama Univ
Nouso, Kazuhiro Okayama Univ
Yagi, Takahito Okayama Univ
Yamamoto, Kazuhide Okayama Univ
抄録
Background: Twist, a transcription factor of the basic helix-loop-helix class, is reported to regulate cancer metastasis. It is known to induce epithelial-mesenchymal transition (EMT). In this study, we evaluated the expression of twist and its effect on cell migration in hepatocellular carcinoma (HCC). Methods: We examined twist expression using immunohistochemistry in 20 tissue samples of hepatocellular carcinoma, and assessed twist expression in HCC cell lines by RT-PCR and Western blot analysis. Ectopic twist expression was created by introducing a twist construct in the twist-negative HCC cell lines. Endogenous twist expression was blocked by twist siRNA in the twist-positive HCC cell lines. We studied EMT related markers, E-cadherin, Vimentin, and N-cadherin by Western blot analysis. Cell proliferation was measured by MTT assay, and cell migration was measured by in vitro wound healing assay. We used immunofluorescent vinculin staining to visualize focal adhesion. Results: We detected strong and intermediate twist expression in 7 of 20 tumor samples, and no significant twist expression was found in the tumor-free resection margins. In addition, we detected twist expression in HLE, HLF, and SK-Hep1 cells, but not in PLC/RPF/5, HepG2, and Huh7 cells. Ectopic twist-expressing cells demonstrated enhanced cell motility, but twist expression did not affect cell proliferation. Twist expression induced epithelial-mesenchymal transition together with related morphologic changes. Focal adhesion contact was reduced significantly in ectopic twist-expressing cells. Twist-siRNA-treated HLE, HLF, and SK-Hep1 cells demonstrated a reduction in cell migration by 50, 40 and 18%, respectively. Conclusion: Twist induces migratory effect on hepatocellular carcinoma by causing epithelial-mesenchymal transition.
発行日
2009-07-18
出版物タイトル
BMC Cancer
9巻
ISSN
1471-2407
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1186/1471-2407-9-240
言語
English
論文のバージョン
publisher
査読
有り
DOI
Web of Sience KeyUT