ID | 53020 |
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著者 |
Kasahara, Kyosuke
Department of Brain Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Miyoshi, Ko
Department of Brain Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Murakami, Shinki
Department of Brain Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Miyazaki, Ikuko
Department of Brain Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
ORCID
Kaken ID
publons
researchmap
Asanuma, Masato
Department of Brain Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
publons
researchmap
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抄録 | In vertebrates, almost all somatic cells extend a single immotile cilium, referred to as a primary cilium. Increasing evidence suggests that primary cilia serve as cellular antennae in many types of tissues by sensing chemical or mechanical stimuli in the milieu surrounding the cells. In rodents an antibody to adenylyl cyclase 3 (AC3) has been widely used to label the primary cilia of neurons in vivo by immunostaining, whereas the lack of markers for the primary cilia of astrocytes has made it difficult to observe astrocytic primary cilia in vivo. Here, we obtained a visualization of astrocytic primary cilia in the mouse brain. In the somatosensory cortex, a large portion of neurons and astrocytes at postnatal day 10 (P10), and of neurons at P56 had AC3-positive primary cilia, whereas only approx. one-half of the astrocytes in the P56 mice carried primary cilia weakly positive for AC3. In contrast, the majority of astrocytes had ADP-ribosylation factor-like protein 13B (Arl13b)-positive primary cilia in the somatosensory cortex and other brain regions of P56 mice. The lengths of astrocytic primary cilia positive for Arl13b varied among the brain regions. Our data indicate that Arl13b is a noteworthy marker of astrocytic primary cilia in the brain.
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キーワード | primary cilia
astrocyte
ADP-ribosylation factor-like protein 13B
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Amo Type | Original Article
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出版物タイトル |
Acta Medica Okayama
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発行日 | 2014-12
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巻 | 68巻
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号 | 6号
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出版者 | Okayama University Medical School
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開始ページ | 317
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終了ページ | 322
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ISSN | 0386-300X
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NCID | AA00508441
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資料タイプ |
学術雑誌論文
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言語 |
英語
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著作権者 | CopyrightⒸ 2014 by Okayama University Medical School
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論文のバージョン | publisher
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査読 |
有り
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