ID | 53010 |
フルテキストURL | |
著者 |
Tsuji, Kenji
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci
Kitamura, Shinji
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci
Kaken ID
publons
Makino, Hirofumi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci
ORCID
Kaken ID
publons
researchmap
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抄録 | The kidneys are exposed to hypoxic conditions during development. Hypoxia-inducible factor (HIF), an important mediator of the response to hypoxia, is believed to have an important role in development. However, the relationship between HIF and branching morphogenesis has not been elucidated clearly. In this study, we examined whether HIF regulates kidney development. We harvested kidneys from day 13 rat embryos (E13K5) and cultured the organs under normoxic (20% 02/5% CO2) or hypoxic (5% 02/5% CO2) conditions. We evaluated the kidneys based on morphology and gene expression. El3K5 cultured under hypoxic conditions had significantly more ureteric bud (UB) branching than the E13Ks cultured under normoxic conditions. In addition, the mRNA levels of GDNF and GDNF receptor (GFR-alpha l), increased under hypoxic conditions in E13K5. When we cultured E13Ks( with the HIF-1 alpha inhibitor digoxin or with siRNA targeting HIF-l alpha under hypoxic conditions, we did not observe increased UB branching. In addition, the expression of GDNF and GFR-alpha 1 was inhibited under hypoxic conditions when the kidneys were treated with siRNA targeting HIF-1 alpha. We also elucidated that hypoxia inhibited UB cell apoptosis and promoted the expression of FGF7 mRNA levels in metanephric mesenchymal (MM) cells in vitro. These findings suggest that hypoxic condition has important roles in inducing branching morphogenesis during kidney development. Hypoxia might mediate branching morphogenesis via not only GDNF/Ret but also FGF signaling pathway.
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キーワード | HIF-I alpha
Kidney development
Hypoxia
Ureteric bud branching
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発行日 | 2014-04-25
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出版物タイトル |
Biochemical and Biophysical Research Communications
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巻 | 447巻
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号 | 1号
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出版者 | Academic Press Inc Elsevier Science
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開始ページ | 108
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終了ページ | 114
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ISSN | 0006-291X
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NCID | AA00564395
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資料タイプ |
学術雑誌論文
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関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/52966
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言語 |
英語
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著作権者 | (c) 2014 Elsevier Inc. All rights reserved.
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論文のバージョン | author
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査読 |
有り
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DOI | |
Web of Science KeyUT |