ID | 53975 |
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著者 |
Yoshii, Taishi
Graduate School of Natural Science and Technology, Okayama University
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Hermann-Luibl, Christiane
Neurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Würzburg
Kistenpfennig, Christa
Graduate School of Natural Science and Technology, Okayama University
Schmid, Benjamin
Max Planck Institute of Molecular Cell Biology and Genetics
Tomioka, Kenji
Graduate School of Natural Science and Technology, Okayama University
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Helfrich-Förster, Charlotte
2Neurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Würzburg
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抄録 | Entrainment to environmental light/dark (LD) cycles is a central function of circadian clocks. In Drosophila, entrainment is achieved by Cryptochrome (CRY) and input from the visual system. During activation by brief light pulses, CRY triggers the degradation of TIMELESS and subsequent shift in circadian phase. This is less important for LD entrainment, leading to questions regarding light input circuits and mechanisms from the visual system. Recent studies show that different subsets of brain pacemaker clock neurons, the morning (M) and evening (E) oscillators, have distinct functions in light entrainment. However, the role of CRY in M and E oscillators for entrainment to LD cycles is unknown. Here, we address this question by selectively expressing CRY in different subsets of clock neurons in a cry-null (cry0) mutant background. We were able to rescue the light entrainment deficits of cry0 mutants by expressing CRY in E oscillators but not in any other clock neurons. Par domain protein 1 molecular oscillations in the E, but not M, cells of cry0 mutants still responded to the LD phase delay. This residual light response was stemming from the visual system because it disappeared when all external photoreceptors were ablated genetically. We concluded that the E oscillators are the targets of light input via CRY and the visual system and are required for normal light entrainment.
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キーワード | circadian clock
clock neurons
Cryptochrome
Drosophila melanogaster
light entrainment
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備考 | This paper is made available under a Creative Commons Attribution 4.0 International (CC BY 4.0) license. https://creativecommons.org/licenses/by/4.0/
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発行日 | 2015-04-15
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出版物タイトル |
The Journal of neuroscience
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巻 | 35巻
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号 | 15号
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出版者 | Society for Neuroscience
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開始ページ | 6131
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終了ページ | 6141
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ISSN | 0270-6474
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NCID | AA10620404
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://dx.doi.org/10.1523/JNEUROSCI.0070-15.2015
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関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/53944
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言語 |
英語
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著作権者 | © 2015 the authors
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論文のバージョン | publisher
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査読 |
有り
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