ID | 32973 |
フルテキストURL | |
著者 |
Fleischmajer, Raul
Mount Sinai School of Medicine, New York
Utani, Atsushi
National Institute of Dental Research, National Institutes of Health
Douglas MacDonald II, E.
Mount Sinai School of Medicine, New York
Perlish, Jerome S
Mount Sinai School of Medicine, New York
Pan, Te-Cheng
Thomas Jefferson University
Chu, Mon-Li
Thomas Jefferson University
Nomizu, Motoyoshi
National Institute of Dental Research, National Institutes of Health
Yamada, Yoshihiko
National Institute of Dental Research, National Institutes of Health
|
抄録 | To study the mechanism of basement membrane formation, we determined by immunochemistry temporal and spatial expression of laminin-5 (Ln-5), laminin-1 (Ln-1) and their integrin receptors during early skin morphogenesis. A 3-dimensional skin culture was used that allows the study of the sequential molecular events of basement membrane formation at the epidermodermal interface. During early anchorage of keratinocytes to the extracellular matrix there is expression of Ln-5, BP-230 antigen and α3, β1 integrin subunits. During epidermal stratification and prior to the formation of the lamina densa there is assembly of Ln-5, Ln-1, collagen IV and nidogen accompanied by keratinocyte basal clustering of α2, α3, α6, β1, and β4 integrin subunits. The assembly pattern of Ln-1 and Ln-5 can be disturbed with functional antibodies against the β1 (AIIB2) and α6 (GoH3) integrin subunits. Ln-1 assembly can also be disturbed with antibodies against its E8 domain and by competitive inhibition with a synthetic peptide (AG-73) derived from its G-4 domain. Quantitative RT-PCR showed that the dermis contributes about 80% of the laminin γ1 chain mRNA while 20% is produced by the epidermis which emphasizes its dual tissue origin and the major contribution of the mesenchyma in laminin production. The laminin γ2 chain mRNA, present in Ln-5, was mostly of epidermal origin. This study presents evidence that during the initiation of basement membrane formation, laminins bind to keratinocyte plasma membrane receptors and thus may serve as nucleation sites for further polymerization of these compounds by a self-assembly process. |
キーワード | Basement membrane
Laminin
Integrin
|
備考 | Published with permission from the copyright holder. This is the institute's copy, as published in Journal of Cell Science, 30 July 1998, Volume 111, Issue 14, 1998, Pages 1929-1940.
Publisher URL:http://jcs.biologists.org/cgi/reprint/111/14/1929 Direct access to Thomson Web of Science record Copyright 1998 © The Company of Biologists Ltd. All rights reserved. |
発行日 | 1998-7
|
出版物タイトル |
Journal of Cell Science
|
巻 | 111巻
|
号 | 14号
|
開始ページ | 1929
|
終了ページ | 1940
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
査読 |
有り
|
Web of Science KeyUT | |
Submission Path | biology_general/7
|