ID | 52009 |
JaLCDOI | |
フルテキストURL | |
著者 |
Katashima, Kazunori
Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kuroda, Masahiro
Radiological Technology, Graduate School of Health Sciences, Okayama University
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Ashida, Masakazu
Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Sasaki, Takanori
Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Taguchi, Takehito
Radiological Technology, Graduate School of Health Sciences, Okayama University
Kaken ID
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Matsuzaki, Hidenobu
Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Murakami, Jun
Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Yanagi, Yoshinobu
Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
ORCID
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Hisatomi, Miki
Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Hara, Marina
Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kato, Hirokazu
Radiological Technology, Graduate School of Health Sciences, Okayama University
Ohmura, Yuichi
Radiological Technology, Graduate School of Health Sciences, Okayama University
Kobayashi, Tomoki
Radiological Technology, Graduate School of Health Sciences, Okayama University
Kanazawa, Susumu
Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
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Harada, Sosuke
Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Takemoto, Mitsuhiro
Department of Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ohno, Seiichiro
Central Division of Radiology, Okayama University Hospital
Mimura, Seiichi
Central Division of Radiology, Okayama University Hospital
Asaumi, Junichi
Department of Oral and Maxillofacial Radiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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抄録 | It is well known that many tumor tissues show lower apparent diffusion coefficient (ADC) values, and that several factors are involved in the reduction of ADC values. The aim of this study was to clarify how much each factor contributes to decreases in ADC values. We investigate the roles of cell density, extracellular space, intracellular factors, apoptosis and necrosis in ADC values using bio-phantoms. The ADC values of bio-phantoms, in which Jurkat cells were encapsulated by gellan gum, were measured
by a 1.5-Tesla magnetic resonance imaging device with constant diffusion time of 30sec. Heating at 42℃ was used to induce apoptosis while heating at 48℃ was used to induce necrosis. Cell death after heating was evaluated by flow cytometric analysis and electron microscopy. The ADC values of bio-phantoms including non-heated cells decreased linearly with increases in cell density, and showed a steep decline when the distance between cells became less than 3μm. The analysis of ADC values of cells after destruction of cellular structures by sonication suggested that approximately two-thirds of the ADC values of cells originate from their cellular structures. The ADC values of bio-phantoms including necrotic cells increased while those including apoptotic cells decreased. This study quantitatively
clarified the role of the cellular factors and the extracellular space in determining the ADC values
produced by tumor cells. The intermediate diffusion time of 30msec might be optimal to distinguish
between apoptosis and necrosis.
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キーワード | ADC
apoptosis
necrosis
hyperthermia
cell density
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Amo Type | Original Article
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出版物タイトル |
Acta Medica Okayama
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発行日 | 2013-12
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巻 | 67巻
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号 | 6号
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出版者 | Okayama University Medical School
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開始ページ | 359
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終了ページ | 367
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ISSN | 0386-300X
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NCID | AA00508441
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資料タイプ |
学術雑誌論文
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言語 |
英語
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著作権者 | CopyrightⒸ 2013 by Okayama University Medical School
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論文のバージョン | publisher
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査読 |
有り
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PubMed ID | |
Web of Science KeyUT |