フルテキストURL NatCommun_12501.pdf
著者 Ohtsuki, Takashi| Kanzaki, Shigeto| Nishimura, Sae| Kunihiro, Yoshio| Sisido, Masahiko| Watanabe, Kazunori|
抄録 The possibility of spatiotemporally photocontrolling translation holds considerable promise for studies on the biological roles of local translation in cells and tissues. Here we report caged aminoacyl-tRNAs (aa-tRNAs) synthesized using a (7-diethylaminocoumarin-4-yl)methoxycarbonyl (DEACM)-cage compound. DEACM-caged aa-tRNA does not spontaneously deacylate for at least 4 h in neutral aqueous solution, and does not bind to the elongation factor Tu. On irradiation at ∼405 nm at 125 mW cm(-2), DEACM-aa-tRNA is converted into active aa-tRNA with a half-life of 19 s. Notably, this rapid uncaging induced by visible light does not impair the translation system. Translation is photoinduced when DEACM-aa-tRNA carrying a CCCG or a CUA anticodon is uncaged in the presence of mRNAs harbouring a CGGG four-base codon or a UAG amber codon, respectively. Protein synthesis is phototriggered in several model systems, including an in vitro translation system, an agarose gel, in liposomes and in mammalian cells.
キーワード Molecular engineering Optogenetics
備考 This is an article published by Nature Publishing Group
発行日 2016-08
出版物タイトル Nature Communications
7巻
開始ページ 12501
ISSN 2041-1723
NCID AA12645905
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン publisher
PubMed ID 27530762
DOI 10.1038/ncomms12501
Web of Sience KeyUT 000381771700001
関連URL https://doi.org/10.1038/ncomms12501
著者 大槻 高史| Miki, Shunya| Kobayashi, Shouhei| Haraguchi, Tokuko| Nakata, Eiji| Hirakawa, Kazutaka| Sumita, Kensuke| 渡邉 和則| Okazaki, Shigetoshi|
発行日 2015-12
出版物タイトル Scientific Reports
5巻
資料タイプ 学術雑誌論文
著者 大槻 高史| Kanzaki, Shigeto| Nishimura, Sae| Kunihiro, Yoshio| Sisido, Masahiko| 渡邉 和則|
発行日 2016-08
出版物タイトル Nature Communications
7巻
資料タイプ 学術雑誌論文