ID | 57482 |
フルテキストURL | |
著者 |
Miyoshi, Yuichi
Graduate School of Natural Science and Technology, Okayama University
Ohtsuki, Takashi
Graduate School of Natural Science and Technology, Okayama University
Kashida, Hiromu
Graduate School of Engineering, Nagoya University
Asanuma, Hiroyuki
Graduate School of Engineering, Nagoya University
Watanabe, Kazunori
Graduate School of Natural Science and Technology, Okayama University
ORCID
Kaken ID
publons
researchmap
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抄録 | Cellular functions are regulated by the up- and down-regulation and localization of RNA molecules. Therefore, many RNA detection methods have been developed to analyze RNA levels and localization. Molecular beacon (MB) is one of the major methods for quantitative RNA detection and analysis of RNA localization. Most oligonucleotide-based probes, including MB, are designed to target a long flexible region on the target RNA molecule, e.g., a single-stranded region. Recently, analyses of tRNA localization and levels became important, as it has been shown that environmental stresses and chemical reagents induce nuclear accumulation of tRNA and tRNA degradation in mammalian cells. However, tRNA is highly structured and does not harbor any long flexible regions. Hence, only a few methods are currently available for detecting tRNA. In the present study, we attempted to detect elongator tRNAMet (eMet) and initiator tRNAMet (iMet) by using an in-stem molecular beacon (ISMB), characterized by more effective quenching and significantly higher sensitivity than those of conventional MB. We found that ISMB1 targeted a 5'- region that includes the D arm of tRNA and that it detected eMet and iMet transcripts as well as mature eMet with high sensitivity. Moreover, the analysis revealed that the formation of the ISMB/tRNA transcript complex required more time than the formation of an ISMB/unstructured short RNA complex. These results suggest that ISMB-based tRNA detection can be a useful tool for various biological and medical studies.
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発行日 | 2019-01-29
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出版物タイトル |
PLoS One
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巻 | 14巻
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号 | 1号
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出版者 | PUBLIC LIBRARY SCIENCE
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開始ページ | e0211505
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ISSN | 1932-6203
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資料タイプ |
学術雑誌論文
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言語 |
フランス語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2019 Miyoshi et al.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1371/journal.pone.0211505
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ライセンス | https://creativecommons.org/licenses/by/4.0/
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Citation | Miyoshi Y, Ohtsuki T, Kashida H, Asanuma H, Watanabe K (2019) In-stem molecular beacon targeted to a 50 -region of tRNA inclusive of the D arm that detects mature tRNA with high sensitivity. PLoS ONE 14(1): e0211505. https://doi. org/10.1371/journal.pone.0211505
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助成機関名 |
日本学術振興会
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助成番号 | 26840010
16H05925
17H05150
18H03933
15K13746
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