このエントリーをはてなブックマークに追加
ID 55452
フルテキストURL
著者
Ohtsuki, Takashi Department of Biomedical Engineering, Okayama University ORCID Kaken ID publons researchmap
Kanzaki, Shigeto Department of Biomedical Engineering, Okayama University
Nishimura, Sae Department of Biomedical Engineering, Okayama University
Kunihiro, Yoshio Department of Biomedical Engineering, Okayama University
Sisido, Masahiko Department of Biomedical Engineering, Okayama University
Watanabe, Kazunori Department of Biomedical Engineering, Okayama University ORCID Kaken ID publons researchmap
抄録
The possibility of spatiotemporally photocontrolling translation holds considerable promise for studies on the biological roles of local translation in cells and tissues. Here we report caged aminoacyl-tRNAs (aa-tRNAs) synthesized using a (7-diethylaminocoumarin-4-yl)methoxycarbonyl (DEACM)-cage compound. DEACM-caged aa-tRNA does not spontaneously deacylate for at least 4 h in neutral aqueous solution, and does not bind to the elongation factor Tu. On irradiation at ∼405 nm at 125 mW cm(-2), DEACM-aa-tRNA is converted into active aa-tRNA with a half-life of 19 s. Notably, this rapid uncaging induced by visible light does not impair the translation system. Translation is photoinduced when DEACM-aa-tRNA carrying a CCCG or a CUA anticodon is uncaged in the presence of mRNAs harbouring a CGGG four-base codon or a UAG amber codon, respectively. Protein synthesis is phototriggered in several model systems, including an in vitro translation system, an agarose gel, in liposomes and in mammalian cells.
キーワード
Molecular engineering
Optogenetics
備考
This is an article published by Nature Publishing Group
発行日
2016-08
出版物タイトル
Nature Communications
7巻
開始ページ
12501
ISSN
2041-1723
NCID
AA12645905
資料タイプ
学術雑誌論文
言語
英語
OAI-PMH Set
岡山大学
著作権者
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン
publisher
PubMed ID
DOI
Web of Science KeyUT
関連URL
https://doi.org/10.1038/ncomms12501