ID | 30447 |
JaLCDOI | |
フルテキストURL | |
著者 |
Hoshida, Yoshihiko
Okayama University
Murakami, Ichiro
Okayama University
Takahashi, Kiyoshi
Okayama University
Akagi, Tadaatsu
Okayama University
|
抄録 | We have attempted to clarify the characteristics of monoclonal antibodies (MAbs) detecting lymphocyte subsets in fixed materials. We examined by means of flow cytometric technique influences of fixatives and reactivity with malignant lymphomas (MLs). Specific markers for T-cells were UCHL1 and OPD4, which reacted especially with helper/inducer T-cells. MT1 recognized almost all of T-cells from peripheral blood and tonsils, but reacted with a part of B-MLs. As for B-cell markers, L26 was the most reliable marker for B-MLs. L26 and MB1 antigens could not be detected on living cells flow cytometrically. LN1 reacted with a part of T-cells as well as B-cells, but fluorescent intensity of the former was apparently stronger than that of the latter. Although LN2 antigen was located mainly in the cytoplasm close to the nuclear membrane immunohistochemically, it could be detected on living cells flow cytometrically. LN2 positive cells belonged to B-cells in peripheral blood and tonsils. When fixed for relatively short time, B5 and buffered formalin were better for examining MAbs than non-buffered formalin and ethanol. |
キーワード | monoclonal antibodies
lymphocyte subset
flow cytometry
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 1990-10
|
巻 | 44巻
|
号 | 5号
|
出版者 | Okayama University Medical School
|
開始ページ | 243
|
終了ページ | 250
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |