ID | 30764 |
JaLCDOI | |
フルテキストURL | |
著者 |
Ino, Hideo
Okayama University
Hayashi, Kazuhiko
Okayama University
Teramoto, Norihiro
Okayama University
Koirala, Tirtha Raj
Okayama University
Chen, Hong-Li
Okayama University
Takahashi, Kiyoshi
Okayama University
Akagi, Tadaastu
Okayama University
|
抄録 | A simian cell line, Si-IIA, harboring Epstein-Barr-virus (EBV) -related herpesvirus (Si-IIA-EBV), produces malignant lymphoma in rabbits when administered by intravenous inoculation. In this study, we analyzed the Si-IIA-EBV genome and compared it with human EBV and herpesvirus macaca fascicularis 1 (HVMF 1 ), which is associated with B-cell lymphoma developing in SIV-infected immunosuppressed monkeys. DNA from Si-IIA-EBV was amplified by the polymerase chain reaction using three different primer pairs complementary to human EBV (B95-8) DNA; two of the primer pairs covered part of the long internal repeat 1 region (IR 1) and the third covered part of the BRRF 1 region. Direct sequencing of the three PCR products revealed that Si-IIA-EBV DNA had about 82% nucleotide homology to the human EBV DNA in all three regions and 92.4% homology to HVMF1 in the IR1 region. The blotting pattern by Southern blot analysis was different between Si-IIA-EBV and human EBV. |
キーワード | Epstein-Barr virus
HVMF 1
lymphoma
?monkey cell line
PCR
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 1997-08
|
巻 | 51巻
|
号 | 4号
|
出版者 | Okayama University Medical School
|
開始ページ | 207
|
終了ページ | 212
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |